University of Texas Brownsville 1 article published in JoVE Biology Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae Miguel F. Gonzales1, Teresa Brooks2, Stefan U. Pukatzki2, Daniele Provenzano1,3 1Department of Biological Sciences, University of Texas Brownsville, 2Department of Medical Microbiology and Immunology, University of Alberta, 3Department of Biomedical Sciences, University of Texas Brownsville Electroporation is a commonly employed method for introducing DNA into bacteria in a process known as transformation. Traditional protocols for the preparation of electrocompetent cells are time consuming and labor intensive. This article describes an alternate, rapid, and efficient method for the preparation of electrocompetent cells presently employed by some laboratories.
