Texas A&M University-College Station 1 article published in JoVE Bioengineering Autofluorescence Imaging to Evaluate Cellular Metabolism Anna Theodossiou1, Linghao Hu1, Nianchao Wang1, Uyen Nguyen1, Alex J. Walsh1 1Department of Biomedical Engineering, Texas A&M University-College Station This protocol describes fluorescence imaging and analysis of the endogenous metabolic coenzymes, reduced nicotinamide adenine (phosphate) dinucleotide (NAD(P)H), and oxidized flavin adenine dinucleotide (FAD). Autofluorescence imaging of NAD(P)H and FAD provides a label-free, nondestructive method to assess cellular metabolism.
