Department of Environmental Quality 1 article published in JoVE Environment Modeling the Size Spectrum for Macroinvertebrates and Fishes in Stream Ecosystems Daniel J. McGarvey1, Taylor E. Woods1,2, Andrew J. Kirk3 1Center for Environmental Studies, Virginia Commonwealth University, 2Department of Ecology and Evolutionary Biology, University of Tennessee, 3Department of Environmental Quality This is a protocol to model the size spectrum (scaling relationship between individual mass and population density) for combined fish and invertebrate data from wadable streams and rivers. Methods include: field techniques to collect quantitative fish and invertebrate samples; lab methods to standardize the field data; and statistical data analysis.