NCI-designated Cancer Center 2 articles published in JoVE Neuroscience Quantification of Immunostained Caspase-9 in Retinal Tissue Crystal K. Colón Ortiz1, Anna M. Potenski2, Kendra V. Johnson1, Claire W. Chen1, Scott J. Snipas3, Ying Y. Jean1, Maria I. Avrutsky*1, Carol M. Troy*1,4,5 1Department of Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University, 2Department of Molecular Pharmacology and Therapeutics, Vagelos College of Physicians and Surgeons, Columbia University, 3NCI-designated Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, 4Department of Neurology, Vagelos College of Physicians and Surgeons, Columbia University, 5The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Vagelos College of Physicians and Surgeons, Columbia University Presented here is a detailed immunohistochemistry protocol to identify, validate, and target functionally relevant caspases in complex tissues. Biology Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes Koen M. O. Galenkamp1, Cheska Marie Galapate1, Yijuan Zhang1, Cosimo Commisso1 1Sanford Burnham Prebys Medical Discovery Institute, NCI-designated Cancer Center Automated assays using multi-well microplates are advantageous approaches for identifying pathway regulators by allowing the assessment of a multitude of conditions in a single experiment. Here, we have adapted the well-established macropinosome imaging and quantification protocol to a 96-well microplate format and provide a comprehensive outline for automation using a multi-mode plate reader.
