University of Windsor View Institution's Website 3 articles published in JoVE Engineering Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering Elizabeth G. Kelley1, Michael H. L. Nguyen2, Drew Marquardt2,3, Brian B. Maranville1, Ryan P. Murphy1 1NIST Center for Neutron Research, National Institute of Standards and Technology, 2Department of Chemistry and Biochemistry, University of Windsor, 3Department of Physics, University of Windsor This protocol presents the use of a stopped-flow sample environment to quickly mix multiple liquid solutions in situ during a small-angle neutron scattering measurement and to study kinetic processes on nanometer length scales and second time scales. Chemistry Preparation and Reactivity of a Triphosphenium Bromide Salt: A Convenient and Stable Source of Phosphorus(I) Stephanie C. Kosnik1, Justin F. Binder1, Maxemilian C. Nascimento1, Charles L. B. Macdonald1 1Department of Chemistry and Biochemistry, University of Windsor The synthesis of a triphosphenium bromide salt is described and its use as a P+ transfer agent is outlined by reactions with an N-heterocyclic carbene and an anionic bisphosphine, yielding an NHC-stabilized P(I) cation and a P(I) containing zwitterion, respectively. Medicine Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen Dennis Ma1, Jonathan Collins2, Tomas Hudlicky2, Siyaram Pandey1 1Department of Chemistry and Biochemistry, University of Windsor, 2Chemistry Department and Centre for Biotechnology, Brock University We have synthesized a novel analogue of pancratistatin with comparable anti-cancer activity as native pancratistatin; interestingly, combinatory treatment with tamoxifen yielded a drastic enhancement in apoptotic and autophagic induction by mitochondrial targeting with minimal effect on noncancerous fibroblasts. Thus, JCTH-4 in combination with tamoxifen could provide a safe anti-cancer therapy.
