University of California, Riverside

8 articles published in JoVE

Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination

JoVE 4430 2/21/2013

Department of Bioengineering, Bourns College of Engineering, University of California, Riverside

A novel method involving quantitative analysis of FRET (Förster Resonance Energy Transfer) signals is described for studying enzyme kinetics. K_M and k_cat were obtained for the hydrolysis of the catalytic domain of SENP1 (SUMO/Sentrin specific protease 1) to pre-SUMO1 (Small Ubiquitin-like MOdifier). The general principles of this quantitative-FRET-based protease kinetic study can be applied to other proteases.

Thinned-skull Cortical Window Technique for In Vivo Optical Coherence Tomography Imaging

JoVE 50053 11/19/2012

Jenny I. Szu¹, Melissa M. Eberle², Carissa L. Reynolds², Mike S. Hsu¹, Yan Wang², Christian M. Oh², M. Shahidul Islam², B. Hyle Park², Devin K. Binder¹

¹Division of Biomedical Sciences, University of California, Riverside, ²Department of Bioengineering, University of California, Riverside

We present a method of creating a thinned-skull cortical window (TSCW) in a mouse model for in vivo OCT imaging of the cerebral cortex.

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

JoVE 4289 9/22/2012

Paul R. Krafft¹, William B. Rolland¹, Kamil Duris¹, Tim Lekic¹, Aaron Campbell², Jiping Tang¹, John H. Zhang^1,3,4

¹Department of Physiology and Pharmacology, Loma Linda University School of Medicine, ²College of Natural and Agricultural Sciences, University of California, Riverside, ³Department of Anesthesiology, Loma Linda University School of Medicine, ⁴Department of Neurosurgery, Loma Linda University School of Medicine

Clinically relevant animal models of intracerebral hemorrhage (ICH) are needed to extend our knowledge of hemorrhagic stroke and to examine novel therapeutic strategies. In this study, we describe and evaluate two ICH models that implement unilateral injections of either autologous whole blood or bacterial collagenase into the basal ganglia (corpus striatum) of mice.

High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.

JoVE 3629 3/12/2012

Hagop S. Atamian, Philip A. Roberts, Isgouhi Kaloshian

Department of Nematology, University of California, Riverside

Two distinct methods to screen plants with root-knot nematodes are described. The described approaches include high-throughput screens with nematodes in a nondestructive manner facilitating the use of these plants in breeding programs.

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

JoVE 3645 3/01/2012

Sonali Chaturvedi¹, Bongsu Jung², Sharad Gupta², Bahman Anvari², A.L.N. Rao¹

¹Department of Plant Pathology and Microbiology, University of California, Riverside, ²Department of Bioengineering, University of California, Riverside

A simple, efficient and robust way to synchronize the delivery of multiple viral components to plant cells via Agrobacterium-mediated transient expression is described. This approach is amenable for studying replication, encapsidation followed by in vitro reassembly of non-viral components into genome depleted optical viral ghosts suitable for biomedical applications.

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

JoVE 3602 11/26/2011

Yuan-Hao Hsu¹, Jolinda A. Traugh²

¹Department of Chemistry, Tunghai University, ²Department of Biochemistry, University of California, Riverside

MALDI-TOF mass spectrometry was successfully utilized to monitor the amide hydrogen/deuterium exchange in protein kinase Pak2 activation.

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

JoVE 3110 10/05/2011

Papri Chatterjee, Yuri Cheung, Chee Liew

UCR Stem Cell Center, Department of Cell Biology and Neuroscience, University of California Riverside

Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

JoVE 780 7/22/2008

Stephane Bertani, Alice Kan, Frank Sauer

Department of Biochemistry, University of California - Riverside

The differentiation of ESC coincides with cell-type specific changes in the structure and composition of chromatin. The detection of those changes provides valuable insights into the mechanisms that define stemcellness and cell differentiation. Chromatin immunoprecipitation (ChIP) represents a valuable method to dissect the molecular mechanisms underlying stem cell differentiation.