Submit
Browse All

The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

Recommend to Librarian
Institution Web Site

Stanford University

45 articles published in JoVE

 JoVE Neuroscience

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster


JoVE 50192 1/25/2013

,

Department of Neurobiology, Stanford University

Genetically encoded optogenetic tools enable noninvasive manipulation of specific neurons in the Drosophila brain. Such tools can identify neurons whose activation is sufficient to elicit or suppress particular behaviors. Here we present a method for activating Channelrhodopsin2 that is expressed in targeted neurons in freely walking flies.

 JoVE Clinical and Translational Medicine

Probe-based Confocal Laser Endomicroscopy of the Urinary Tract: The Technique


JoVE 4409 1/10/2013

1,2, 1,2, 1,2

1Department of Urology, Stanford University School of Medicine, 2Veterans Affairs Palo Alto Health Care System

Probe-based confocal laser endomicroscopy enables real-time microscopy of the human urinary tract during cystoscopy, providing dynamic, intravital imaging of pathological states such as bladder cancer with cellular resolution. Endomicroscopy may augment the diagnostic accuracy of standard white light endoscopy and provide intraoperative image guidance to improve surgical resection.

 JoVE Clinical and Translational Medicine

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring


JoVE 4341 11/13/2012

, , , ,

Department of Radiology and Bio-X Program Canary Cancer at Stanford for Cancer Early Detection, Stanford University

Use of Cerenkov Luminescence Imaging (CLI) for monitoring preclinical cancer treatment is described here. This method takes advantage of Cerenkov Radiation (CR) and optical imaging (OI) to visualize radiolabeled probes and thus provides an alternative to PET in preclinical therapeutic monitoring and drug screening.

 JoVE Clinical and Translational Medicine

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate


JoVE 4221 10/31/2012

*1,2, *1,3, 1, 1, 1, 1,4, 5, 1, 1

1Department of Surgery, Stanford University, 2Department of Surgery, Duke University, 3Department of Surgery, Saint Joseph Mercy Hospital, 4School of Medicine, University of California, San Francisco, 5School of Dentistry, University of California, Los Angeles

This protocol describes the isolation of adipose-derived stromal cells from lipoaspirate and the creation of a 4 mm critical-sized calvarial defect to evaluate skeletal regeneration.

 JoVE Clinical and Translational Medicine

Collecting And Measuring Wound Exudate Biochemical Mediators In Surgical Wounds


JoVE 50133 10/20/2012

, , ,

Department of Anesthesia, Stanford University School of Medicine

This article provides a detailed and visual description of a methodology for collecting and measuring biochemical inflammatory and nociceptive mediators at the surgical wound site following cesarean delivery. This human bioassay has been used to determine correlations between wound and serum cytokine concentrations and drug-mediated changes in wound cytokines, chemokines and neuropetides.

 JoVE General

Synthesis of an In vivo MRI-detectable Apoptosis Probe


JoVE 3775 7/31/2012

1, 2,3, 1, 1

1Division of Cardiovascular Medicine, Department of Medicine, Stanford University Medical Center, 2Division of Cardiology, Department of Medicine, University of California, San Francisco, 3San Francisco VAMC

Early detection of apoptosis may identify at-risk cell populations in a variety of diseases. Here we demonstrate a method to link an early apoptosis-detection protein (Annexin V) to a MRI-detectable iron oxide nanoparticle (SPIO). This method may be extended to other proteins of interest to generate MRI-detectable molecular imaging probes.

 JoVE Neuroscience

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI


JoVE 4055 7/12/2012

1, 2, 3, 3, 2

1Department of Radiology, Stanford University, 2Center for In Vivo Microscopy, Duke University Medical Center, 3Department of Biomedical Engineering, Duke University

A technique is described for broadly opening the blood-brain barrier in the mouse using microbubbles and ultrasound. Using this technique, manganese can be administered to the mouse brain. Because manganese is an MRI contrast agent that accumulates in depolarized neurons, this approach enables imaging of neuronal activity.

 JoVE Bioengineering

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip


JoVE 4290 7/07/2012

1, 2,3,4, 1, 1, 2,3, 1, 1

1Department of Plant Biology, Carnegie Institution for Science, 2Howard Hughes Medical Institute, 3Departments of Applied Physics and Bioengineering, Stanford University, 4Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS), University of Freiburg

This article provides a protocol for cultivation of Arabidopsis seedlings in the RootChip, a microfluidic imaging platform that combines automated control of growth conditions with microscopic root monitoring and FRET-based measurement of intracellular metabolite levels.

 JoVE Clinical and Translational Medicine

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis


JoVE 3663 5/09/2012

1,2, 1,2, 3, 3, 4, 4, 5, 5, 6, 1,2, 7, 1,2,7

1University Heart Center Hamburg, TSI-Lab, Germany, 2Cardiovascular Research Center, University of Hamburg, 3Department of Medicine, Cardiology Division, Pulmonary Hypertension Program, University of Alberta, 4Department of Medicine, Stanford University School of Medicine, 5Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics, University of Veterinary Medicine, Vienna, 6Translumina GmbH, Hechingen, 7Department of Cardiothoracic Surgery, Stanford University School of Medicine

This video shows a model to study the development of intimal hyperplasia after stent deployment using a human vessel (IMA) in an immunodeficient rat model.

 JoVE Bioengineering

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids


JoVE 3890 3/02/2012

, , ,

Mechanical Engineering, Stanford University

Isotachophoresis (ITP) is a robust electrokinetic separation and preconcentration technique with applications ranging from toxin detection to sample preparation. We review the physical principles of ITP and the methodology of applying this technique to two specific example applications: separation and detection of small molecules and purification of nucleic acids from cell culture lysate.

 JoVE Neuroscience

Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry


JoVE 3432 12/23/2011

, , ,

Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine

Flow cytometry is a powerful tool allowing for the isolation and study of specific cell populations. This protocol describes steps for isolating LacZ-expressing cells from cochlear tissues from neonatal transgenic mice. Dissociated cochlear cells were labeled using fluorescent-conjugated substrates of β-galactosidase prior to separation via flow cytometry.

 JoVE Clinical and Translational Medicine

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle


JoVE 3482 11/04/2011

1,2, 1,2, 1,2, 1

1Department of Radiology, Molecular Imaging Program at Stanford (MIPS), 2Stanford School of Medicine, Stanford University

We describe a technique for labeling and tracking stem cells with FDA-approved, superparamagnetic iron oxide (SPIO), ferumoxytol (Feraheme). This cellular imaging technique that utilizes magnetic resonance (MR) imaging for visualization, is readily accessible for long-term monitoring and diagnosis of successful or unsuccessful stem cell engraftments in patients.

 JoVE General

Competitive Genomic Screens of Barcoded Yeast Libraries


JoVE 2864 8/11/2011

*1,2, *2,3, *4, 1,2, 5, 2,3, 2,6, 1,2,3

1Banting and Best Department of Medical Research and Department of Molecular Genetics, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 3Donnelly Sequencing Centre, University of Toronto, 4Genetics and Molecular Biology Branch, National Human Genome Research Institute, NIH, 5Stanford Genome Technology Center, Stanford School of Medicine, Stanford University, 6Department of Pharmaceutical Sciences, University of Toronto

We have developed comprehensive, unbiased genome-wide screens to understand gene-drug and gene-environment interactions. Methods for screening these mutant collections are presented.

 JoVE Bioengineering

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)


JoVE 2605 6/01/2011

1,2, 1, 1, 1,3, 2, 2, 1, 3, 2, 1,3

1Transplant and Stem Cell Immunobiology Lab (TSI) and CVRC, University Hospital Hamburg, University Heart Center Hamburg, 2Department of Experimental and Clinical Pharmacology and Toxicology, University Heart Center Hamburg, 3CT Surgery, Stanford University School of Medicine

This video demonstrates the use of in vivo bioluminescence imaging to study immune responses after implantation of Engineered Heart Tissue (EHT) in rats.

 JoVE General

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran


JoVE 2672 4/28/2011

1, 2, 2, 1

1Department of Biological Sciences, Vanderbilt University, 2Department of Chemical and Systems Biology, Stanford University

This protocol delineates a way to label and trace the fate of small groups of cells zebrafish embryos using UV-uncaging of caged fluorescein, followed by whole mount immunolabeling to amplify the signal from the uncaged fluorescein.

 JoVE General

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA


JoVE 2593 4/26/2011

1, 2, 3, 2, 3

1Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 2Department of Molecular and Cell Biology, University of Connecticut, 3Department of Pharmaceutical Sciences, University of Connecticut

Nucleosome ELISA (NU-ELISA) is a sensitive and quantitative method to detect global patterns of post-translational modifications in preparations of native, intact nucleosomes. These modifications include methylations, acetylations, and phosphorylations at specific histone amino acid residues, and hence NU-ELISA provides a global proteomic assay of the overall chromatin modification states of specific cell types.

 JoVE General

Associated Chromosome Trap for Identifying Long-range DNA Interactions


JoVE 2621 4/23/2011

,

Medical Service, VA Palo Alto Health Care System , Stanford University School of Medicine

The associated chromosome trap (ACT) assay is a novel unbiased method for identifying long-range DNA interactions. The characterization of long range DNA interactions will allow us to determine the relationship of nuclear architecture to gene expression in both normal physiology and in diseased states.

 JoVE General

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses


JoVE 2498 2/25/2011

1, 1, 2, 1

1Department of Biological Sciences, University of Alabama Huntsville, 2Department of Biology, Stanford University

A major impediment to biochemical analyses of ribosomes containing nascent peptidyl-tRNAs has been the presence of other ribosomes in the same samples, ribosomes not involved in the translation of the specific mRNA sequence being analyzed. We developed a simple methodology to purify, exclusively, the ribosomes containing the nascent peptidyl-tRNA of interest.

 JoVE Clinical and Translational Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy


JoVE 1989 12/04/2010

1, 1,2, 1, 2, 1, 1,2

1University Heart Center Hamburg, Transplant and Stem Cell Immunobiology Lab (TSI), University Hospital Hamburg, 2Stanford University School of Medicine

This video demonstrates the orthotopic aortic transplant model as a simple model to study the development of transplant vasculopathy (TVP) in rats.

 JoVE General

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease


JoVE 1437 1/20/2010

1, 1,2, 1,2,3, 3, 1,2, 1,2,3

1Transplant and Stem Cell Immunobiology Lab (TSI), University Heart Center Hamburg, 2CVRC, University Hospital Hamburg, 3Department of CT Surgery, Stanford University School of Medicine

This video shows and compares two experimental models to study the development of obliterative airway disease (OAD) in mice, the heterotopic and orthotopic tracheal transplantation model.

 JoVE General

LAD-Ligation: A Murine Model of Myocardial Infarction


JoVE 1438 10/14/2009

1,2, 1,2, 1,2, 1,2,3, 3, 1,2, 1,2,3

1Transplant and Stem Cell Immunobiology Lab (TSI), University Heart Center Hamburg, 2CVRC, University Hospital Hamburg, 3Department of CT Surgery, Stanford University School of Medicine

This video demonstrates how to use a fast and reliable model to study pathobiological and pathophysiological processes of myocardial ischemia.

 JoVE General

Imaging In-Stent Restenosis: An Inexpensive, Reliable, and Rapid Preclinical Model


JoVE 1346 9/14/2009

1, 2, 1, 1

1Department of Cardiothoracic Surgery, Stanford University School of Medicine, 2Stanford University School of Medicine

This video demonstrates how to use a preclinical inexpensive and reliable model to study pathobiological and pathophysiological processes of in-stent restenosis development. Longitudinal in vivo monitoring using OCT (Optical Coherence Tomography) and analysis of OCT images are also demonstrated.

 JoVE General

Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase


JoVE 1280 7/23/2009

1, 2, 2, 1

1Department of Cardiovascular Medicine, Stanford University School of Medicine, 2Department of Vascular Surgery, Stanford University School of Medicine

This video shows how to induce abdominal aortic aneurysms (AAA) in mice via transient intraluminal infusion of porcine pancreatic elastase into the infrarenal segment of the abdominal aorta. The model has the ability to add broad insight into the pathobiology of AAA due to the emergence of numerous transgenic and gene knockout mice.

 JoVE General

Calcium Imaging of Cortical Neurons using Fura-2 AM


JoVE 1067 1/19/2009

1, 2

1Department of Neurobiology, Stanford University, 2Department of Neurobiology, Stanford University School of Medicine

Calcium signals play a key role in many cellular processes including gene expression, survival and differentiation. Here we demonstrate how to perform calcium imaging using Fura-2 AM. Calcium imaging is a valuable tool to study the regulation of intracellular calcium in real time and its regulation of signaling cascades.

 JoVE General

Determining heat and mechanical pain threshold in inflamed skin of human subjects


JoVE 1092 1/14/2009

, , ,

Department of Anesthesia, Stanford University School of Medicine

Algorithms assessing heat and mechanical pain thresholds in experimentally inflamed skin of human study subjects are shown. The two pain testing paradigms independently examine nociceptive processing by the two major peripheral nerve fiber populations transmitting pain, i.e., non-myelinated C fibers and small myelinated A-delta fibers.

 JoVE General

Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators


JoVE 1074 12/01/2008

1, 1, 2,3, 1, 1

1Department of Anesthesia, Stanford University School of Medicine, 2Department of Anaesthesiology, University of Mannheim, 3Department of Anaesthesiology, University of Heidelberg

A technique is presented for the in-vivo collection of interstitial fluid samples from pertinent tissue sites (here, experimentally inflamed skin) for the measurement of biochemicals mediating pain and inflammation.

 JoVE General

Pressure-polishing Pipettes for Improved Patch-clamp Recording


JoVE 964 10/22/2008

, ,

Department of Molecular and Cellular Physiology, Stanford University School of Medicine

This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.

 JoVE General

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes


JoVE 936 10/16/2008

1, 2, 2

1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Molecular and Cellular Physiology, Stanford University School of Medicine

This is intended as an introduction to patch clamp recording from Xenopus laevis oocytes. It covers vitelline membrane removal, formation of a gigaohm seal (gigaseal), and the optional conversion of the patch to the outside-out topology.

 JoVE General

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller


JoVE 939 10/08/2008

1, 2, 2

1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Molecular and Cellular Physiology, Stanford University School of Medicine

This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.

 JoVE General

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging


JoVE 827 8/03/2008

,

Division of Cardiovascular Medicine, Stanford University

In this video, we are showing how to label human embryonic stem cells (hESC) with manganese chloride (MnCl2) which can enter cells via voltage-gated calcium channels when the cells are biologically active. Additionally, we show the use of MnCl2 as cellular MRI contrast agent to determine the in vitro viability of hESC.

 JoVE General

In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells


JoVE 740 5/02/2008

, , ,

Departments of Radiology and Medicine (Cardiology), Stanford University School of Medicine

With the growing interest in stem cell therapies, molecular imaging techniques are ideal for monitoring stem cell behavior after transplantation. Luciferase reporter genes have enabled non-invasive, repetitive assessment of cell survival, location, and proliferation in vivo. This video will demonstrate how to track hESC proliferation in a living mouse.

 JoVE General

Orthotopic Mouse Model of Colorectal Cancer


JoVE 484 12/04/2007

1,2, 2, 2

1Department of Surgery, University of California, San Francisco - UCSF, 2Department of Pathology, Stanford University School of Medicine

Two techniques can be used to establish this model: injection of a cancer cell suspension into the cecal wall or transplantation of a piece of subcutaneous tumor onto the cecum. This model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.

Waiting
simple hit counter