Children's Hospital of Chicago View Institution's Website 1 article published in JoVE Neuroscience In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development Ben Yang*1, Lauren B. Geary*1, Yong-Chao Ma1,2 1Northwestern University Feinberg School of Medicine, Children's Hospital of Chicago Research Center, 2Departments of Pediatrics, Neurology and Physiology, Northwestern University Feinberg School of Medicine To assess the function and the regulation of genes during the development of midbrain dopaminergic neurons, we describe a method that involves in ovo electroporation of plasmid DNA constructs into embryonic chick ventral midbrain dopaminergic neuron progenitors. This technique can be used to achieve efficient expression of genes of interest to study different aspects of midbrain development and dopaminergic neuron differentiation.