Purdue University

13 articles published in JoVE

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

JoVE 50020 4/18/2013

Wan W. Amani Wan Salim¹, Michael A. Zeitchek¹, Andrew C. Hermann¹, Antonio J. Ricco², Ming Tan², Florian Selch², Erich Fleming², Brad M. Bebout², Mamoun M. Bader³, Aeraj ul Haque⁴, D. Marshall Porterfield⁵

¹Department of Agricultural and Biological Engineering, Birck-Bindley Physiological Sensing Facility, Purdue University, ²NASA Ames Research Center, ³Department of Chemistry, Pennsylvania State University Hazleton, ⁴Cooley LLP, ⁵NASA Life and Physical Sciences, Human Exploration and Operations Mission Directorate, NASA Headquarters

All-solid-state ion-selective electrodes (ASSISEs) constructed from a conductive polymer (CP) transducer provide several months of functional lifetime in liquid media. Here, we describe the fabrication and calibration process of ASSISEs in a lab-on-a-chip format. The ASSISE is demonstrated to have maintained a near-Nernstian slope profile after prolonged storage in complex biological media.

Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue

JoVE 50126 2/11/2013

Andrew J. Woolley¹, Himanshi A. Desai¹, Janak Gaire², Andrew L. Ready¹, Kevin J. Otto^1,2

¹Weldon School of Biomedical Engineering, Purdue University, ²Department of Biological Sciences, Purdue University

Here we present a histological method for capturing, labeling, optically clearing, and imaging the intact brain tissue interface around chronically implanted microdevices in rodent brain tissue. Results from the techniques comprising this method are useful for understanding the impact of various penetrating brain-implants on their surrounding tissue.

Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices

JoVE 50034 10/29/2012

Staci E. Engle, Hilary J. Broderick, Ryan M. Drenan

Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University

In this paper, we describe a useful method to study ligand-gated ion channel function in neurons of acutely isolated brain slices. This method involves the use of a drug-filled micropipette for local application of drugs to neurons recorded using standard patch clamp techniques.

Investigation of Early Plasma Evolution Induced by Ultrashort Laser Pulses

JoVE 4033 7/02/2012

Wenqian Hu, Yung C. Shin, Galen B. King

Mechanical Engineering, Purdue University

An experimental method to examine the early plasma evolution induced by ultrashort laser pulses is described. Using this method, high quality images of early plasma are obtained with high temporal and spatial resolutions. A novel integrated atomistic model is used to simulate and explain the mechanisms of early plasma.

Voltage Biasing, Cyclic Voltammetry, & Electrical Impedance Spectroscopy for Neural Interfaces

JoVE 3566 2/24/2012

Seth J. Wilks¹, Tom J. Richner², Sarah K. Brodnick², Daryl R. Kipke³, Justin C. Williams², Kevin J. Otto^1,4

¹Weldon School of Biomedical Engineering, Purdue University, ²Biomedical Engineering, University of Wisconsin-Madison, ³Biomedical Engineering, University of Michigan, ⁴Department of Biological Sciences, Purdue University

The electrode-tissue interface of neural recording electrodes can be characterized with electrical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Application of voltage biasing changes the electrochemical properties of the electrode-tissue interface and can improve recording capability. Voltage biasing, EIS, CV, and neural recordings are complementary.

Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays

JoVE 3600 12/20/2011

Kristen N. Fantetti, Donna M. Fekete

Department of Biological Sciences, Purdue University

We demonstrate how to dissect and culture chick E4 statoacoustic ganglion and E6 spinal cord explants. Explants are cultured under serum-free conditions in 3D collagen gels for 24 hours. Neurite responsiveness is tested with growth factor-supplemented medium and with protein-coated beads.

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells

JoVE 2186 10/06/2010

Sophie Moreau-Marquis¹, Carly V. Redelman², Bruce A. Stanton¹, Gregory G. Anderson²

¹Department of Physiology, Dartmouth College, ²Department of Biology, Indiana University Purdue University Indianapolis

This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.

Microdissection of Zebrafish Embryonic Eye Tissues

JoVE 2028 6/27/2010

Liyun Zhang, Yuk Fai Leung

Department of Biological Sciences, Purdue University

This article describes an approach to microdissect zebrafish retinas with and without retinal pigment epithelium attached, from one to three days postfertilization embryos.

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

JoVE 1863 3/24/2010

Debarati Mukherjee, Arpita Sen, R. Claudio Aguilar

Department of Biological Sciences and Purdue Center for Cancer Research, Purdue University

Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of phenotype development as a function of protein concentration at population and single-cell levels in Saccharomyces cerevisiae.

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

JoVE 1398 10/01/2009

Eric Johansen¹, Birgit Schilling², Michael Lerch¹, Richard K. Niles¹, Haichuan Liu¹, Bensheng Li², Simon Allen¹, Steven C. Hall¹, H. Ewa Witkowska¹, Fred E. Regnier³, Bradford W. Gibson², Susan J. Fisher¹, Penelope M. Drake¹

¹Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco - UCSF, ²Buck Institute for Age Research, ³Department of Chemistry, Purdue University

Lectin-conjugated POROS beads were employed for HPLC. Glycopeptide standards served as positive and negative controls. MARS-14 depleted, trypsin-digested human plasma was chromatographed and flow-through (FT) and bound fractions collected for ESI-LC-MS/MS analyses. Glycopeptides were enriched in the bound fraction as compared to FT.

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

JoVE 1471 8/10/2009

Samuel M. Peterson, Jennifer L. Freeman

School of Health Sciences, Purdue University

Gene microarrays are powerful tools in gene expression profiling at a genome-wide level. This technology has application in a variety of biological disciplines including developmental biology and toxicology. In this video, we detail a protocol for global gene expression analysis using a comprehensive oligonucleotide microarray platform for the zebrafish.

RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis

JoVE 1470 8/07/2009

Samuel M. Peterson, Jennifer L. Freeman

School of Health Sciences, Purdue University

The isolation of high quality, intact RNA is an essential step in many laboratory protocols. Here, we demonstrate RNA extraction from whole zebrafish embryos and cDNA synthesis for subsequent application in various experimental procedures including gene expression microarray analysis.

Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones

JoVE 662 2/20/2008

Aih Cheun Lee, Boris Decourt, Daniel M. Suter

Department of Biological Sciences, Purdue University

Aplysia californica neurons develop large growth cones in culture that are excellent for high-resolution imaging of growth cone motility and guidance. Here, we present a protocol for dissection and plating of Aplysia bag cell neurons as well as for setting up a chamber for live cell imaging.