JoVE   
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  JoVE Biology

  
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  JoVE Neuroscience

  
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  JoVE Immunology and Infection

  
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  JoVE Clinical and Translational Medicine

  
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  JoVE Bioengineering

  
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  JoVE Applied Physics

  
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  JoVE Chemistry

  
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  JoVE Behavior

  
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  JoVE Environment

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JoVE Science Education

General Laboratory Techniques

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Basic Methods in Cellular and Molecular Biology

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Model Organisms I

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Model Organisms II

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Institution Web Site

Louisiana State University

13 articles published in JoVE

 JoVE Biology

Chromosome Preparation From Cultured Cells

1Department of Genetics, Louisiana State University Health Science Center


JoVE 50203

Chromosomes can be isolated from live cells such as lymphocytes or skin fibroblasts, and from organisms including humans or mice. These chromosome preparations can be further utilized for routine G-banding and molecular cytogenetic procedures such as fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and spectral karyotyping (SKY).

 JoVE Bioengineering

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies

1Center for Advanced Microstructures and Devices (CAMD), Louisiana State University, 2Center for Atomic-Level Catalyst Design, Cain Department of Chemical Engineering, Louisiana State University, 3Department of Biological and Agricultural Engineering, Louisiana State University, 4Argonne National Laboratory


JoVE 50711

Millifluidic devices are utilized for controlled synthesis of nanomaterials, time-resolved analysis of reaction mechanisms and continuous flow catalysis.

 JoVE Immunology and Infection

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay

1Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, 2Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, 3Department of Microbiology and Immunology, SUNY Upstate Medical University, 4Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center


JoVE 4165

The phagokinetic motility track assay is a method used to assess the movement of cells. Specifically, the assay measures chemokinesis (random cell motility) over time in a quantitative manner. The assay takes advantage of the ability of cells to create a measurable track of their movement on colloidal gold-coated coverslips.

 JoVE Neuroscience

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

1Department of Pharmacology, Toxicology, & Neuroscience, Louisiana State University Health Sciences Center


JoVE 4171

Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in rodents.

 JoVE Neuroscience

Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol

1LSU Health Sciences Center - New Orleans, 2Medical School and Stanley S. Scott Cancer Center


JoVE 3965

We describe a rapid methodology to isolate and culture hippocampal and cortical neurons from rodent embryos. This protocol allows us to perform experiments in which nearly pure neuronal cultures are required.

 JoVE Biology

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

1Department of Biochemistry and Molecular Biology, LSU School of Medicine, 2Department of Oral Biology, LSU School of Dentistry, 3Stanley S. Scott Cancer Center, LSU School of Medicine


JoVE 3585

This method allows monitoring of cells in real time and quantitative measurements of different cell migration parameters such as speed, displacement, and velocity. Unlike the traditional methods, this real time approach is not based on endpoint quantitative migration measurements; instead it allows monitoring and calculating different parameters continuously.

 JoVE Neuroscience

Methods to Assay Drosophila Behavior

1Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, 2Department of Genetics, Louisiana State University Health Sciences Center


JoVE 3795

Drosophila melanogaster is a genetically and behaviorally tractable model system that has been used to understand the molecular and cellular basis of many important biological processes for over a century 1. Drosophila has been well exploited to gain insights into the genetic basis of fly behavior.

 JoVE Immunology and Infection

Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics

1Department of Physiology, School of Medicine, Louisiana State University Health Sciences Center


JoVE 3438

We introduce an approach to evaluate the cytosolic Ca2+ concentration in isolated lymphatics to study Ca2+-dependent and Ca2+-sensitizing mechanisms of lymphatic smooth muscle contraction.

 JoVE Immunology and Infection

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

1Louisiana State University Health Sciences Center


JoVE 3382

Key techniques to be used in the evaluation of Candida vaginitis in an experimental animal model are described. The methods will allow rapid collection of vaginal specimens and lymphocytes from draining lumbar lymph nodes. These techniques could give rise to mouse models of other diseases in the female lower genital tract.

 JoVE Biology

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin

1Department of Physiology, Louisiana State University Health Sciences Center


JoVE 3187

Microscopic imaging of live endothelial cells expressing GFP-actin allows characterization of dynamic changes in cytoskeletal structures. Unlike techniques that use fixed specimens, this method provides a detailed assessment of temporal changes in the actin cytoskeleton in the same cells before, during, and after various physical, pharmacological, or inflammatory stimuli.

 JoVE Biology

Bioassays for Monitoring Insecticide Resistance

1Division of Plant Sciences, University of Missouri, Delta Research Center, 2Department of Entomology, Louisiana State University Agricultural Center


JoVE 2129

This manuscript demonstrates and discusses techniques used to survey pesticide susceptibility and detect resistance to contact and systemic pesticides in arthropod pests.

 JoVE Biology

Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)

1Department of Entomology, Louisiana State University Agricultural Center


JoVE 2190

We present procedures for demonstrating that ligands bind to the surface membrane of the cellulose-digesting protozoa in the gut of Formosan subterranean termites using fluorescent microscopy and that ligands coupled with lytic peptides kill these protozoa in vitro (anaerobic protozoa culture) and in vivo (injection into the termite hindgut).

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