Immuno Biological Laboratories Co., Ltd. View Institution's Website 1 article published in JoVE Immunology and Infection A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor Shinya Urano1, Ryushi Tazawa1, Takahito Nei1, Natsuki Motoi1, Masato Watanabe2, Takenori Igarashi3, Masahiro Tomita3, Koh Nakata1 1Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, 2First department of Internal Medicine, School of Medicine, Kyorin University, 3Neosilk Laboratory, Immuno Biological Laboratories Co., Ltd. We designed a cell-free receptor binding assay in order to estimate the binding of granulocyte-macrophage colony-stimulating factor (GM-CSF) to the receptors. It enables us to evaluate competitive inhibition of biotinylated GM-CSF binding to soluble GM-CSF receptor alpha by GM-CSF autoantibody with excellent reproducibility.
