1Department of Biological Sciences, Ohio University
"Freeze-cracking," a method for exposing the inner tissues of the nematode C. elegans to antibodies for protein localization, is demonstrated.
Published October 14, 2013. Keywords: Molecular Biology, Caenorhabditis elegans (C. elegans), Fluorescent Antibody Technique, nematode, labeling, localization, in situ
1Institute of Biochemistry, Food Science, and Nutrition , The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, 2Department of Physics and Astronomy, Ohio University
Ice binding proteins (IBPs), also known as antifreeze proteins, inhibit ice growth and are a promising additive for use in the cryopreservation of tissues. The main tool used to investigate IBPs is the nanoliter osmometer. We developed a home-designed cooling stage mounted on an optical microscope and controlled using a custom-built LabVIEW routine. The nanoliter osmometer described here manipulated the sample temperature in an ultra-sensitive manner.
Published February 4, 2013. Keywords: Biochemistry, Chemistry, Physics, Biophysics, Bioengineering, Microbiology, Proteins, Ice binding proteins, IBP, antifreeze proteins, thermal hysteresis proteins, TH, ice structuring proteins, recrystallization inhibition proteins, nanoliter osmometer, LabVIEW, temperature control, microscopy stage, nano, Gibbs-Thomson effect, microfluidics, microscale chemistry
1Ohio Musculoskeletal and Neurological Institute (OMNI) and the Department of Biomedical Sciences, Ohio University
Transcranial magnetic stimulation (TMS) is a non-invasive tool to gain insight on the physiology and function of the human nervous system. Here, we present our TMS techniques to study cortical excitability of the upper limb and lumbar musculature.
Published January 20, 2012. Keywords: Medicine, neuroscience, muscle, electromyography, physiology, TMS, strength, motor control. sarcopenia, dynapenia, lumbar
JoVE Immunology and Infection
1Molecular and Cell Biology Program, Ohio University, 2Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, 3Department of Biomedical Engineering, Russ College of Engineering and Technology, Ohio University
Dendritic cells uptake antigens and migrate towards immune organs to present processed antigens to T cells. Qdot nanocrystal labeling provides a long-lasting and stable fluorescent signal. This allows tracking of dendritic cells to different organs by fluorescent microscopy.
Published June 2, 2011. Keywords: Immunology, Dendritic cells, Qdot nanocrystals, labeling, cell tracking, mouse