University of Toledo View Institution's Website 9 articles published in JoVE Bioengineering A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation Qingliang Yang1, Jordan C. Langston2, Yuan Tang3, Balabhaskar Prabhakarpandian4, Laurie E. Kilpatrick5, Mohammad F. Kiani1,2,6 1Department of Mechanical Engineering, Temple University, 2Department of Bioengineering, Temple University, 3Department of Bioengineering, University of Toledo, 4Biomedical Technology, CFD Research Corporation, 5Center for Inflammation and Lung Research, Department of Microbiology, Immunology and Inflammation, Lewis Katz School of Medicine, Temple University, 6Department of Radiation Oncology, Lewis Katz School of Medicine, Temple University In this protocol, a biomimetic microfluid assay, which can reproduce a physiologically relevant microvascular environment and reproduce the entire leukocyte adhesion/migration cascade, is employed to study leukocyte-endothelial cell interactions in inflammatory disease. Biochemistry Reconstitution of Msp1 Extraction Activity with Fully Purified Components Heidi L. Fresenius1, Matthew L. Wohlever1 1Department of Chemistry & Biochemistry, University of Toledo Here, we present a detailed protocol for reconstitution of Msp1 extraction activity with fully purified components in defined proteoliposomes. Chemistry Achieving Moderate Pressures in Sealed Vessels Using Dry Ice As a Solid CO2 Source Mohit Kapoor1,2, Pratibha Chand-Thakuri*1,2, Justin M. Maxwell*1,2, Michael C. Young1,2 1Department of Chemistry and Biochemistry, University of Toledo, 2School of Green Chemistry and Engineering, University of Toledo Here we present a protocol for performing reactions in simple reaction vessels under low-to-moderate pressures of CO2. The reactions can be performed in a variety of vessels simply by administering the carbon dioxide in the form of dry ice, without the need for costly or elaborate equipment or set-ups. Neuroscience Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain Alzahra J. Al Omran1, Hannah C. Saternos1, Tongyu Liu2, Surya M. Nauli3, Wissam A. AbouAlaiwi1 1Department of Pharmacology and Experimental Therapeutics, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, 2Life Sciences Institute, University of Michigan, 3Department of Biomedical & Pharmaceutical Sciences, Chapman University, School of Pharmacy, Rinker Health Science campus Using high-resolution differential interference contrast (DIC) microscopy, an ex vivo observation of the beating of motile ependymal cilia located within the mouse brain ventricles is demonstrated by live-imaging. The technique allows a recording of the unique ciliary beating frequency and beating angle as well as their intracellular calcium oscillation pacing properties. Biology Imaging Centrosomes in Fly Testes Marcus L. Basiri1, Stephanie Blachon1, Yiu-Cheung Frederick Chim1, Tomer Avidor-Reiss1 1Department of Biological Sciences, University of Toledo Imaging of centrosomal proteins during Drosophila spermatogenesis is a powerful method to identify new proteins critical for centrosome biology as well as to elucidate the particular function of known players in this process. Neuroscience Experimental Methods for Testing the Effects of Neurotrophic Peptide, ADNF-9, Against Alcohol-induced Apoptosis during Pregnancy in C57BL/6 Mice Youssef Sari1 1Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo The experimental designs proposed here focus on studying the effects of alcohol exposure in apoptosis and the application of neurotrophic peptide during pregnancy in fetal brain. A detailed description from the breeding to the collection of fetal brains is described. Techniques for determination of apoptosis are also described in detail. Neuroscience Automated Quantification of Synaptic Fluorescence in C. elegans Brianne L. Sturt1, Bruce A. Bamber1 1Department of Biological Sciences, University of Toledo The abundance of neurotransmitter receptors clustered at synapses strongly influences synaptic strength. This method quantifies fluorescently-labeled neurotransmitter receptors in three dimensions with single-synapse resolution in C. elegans, allowing hundreds of synapses to be rapidly characterized within a single sample without distortions introduced by z-plane projection. Medicine Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease Wissam A. AbouAlaiwi1, Ingrid Rodriguez2, Surya M. Nauli1 1Department of Pharmacology, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, 2Department of Emergency and Intensive Care, ProMedica Sponsored Research Spectral Karyotyping (SKY) is an advanced cytogenetics technique to identify genomic and chromosomal aberrations. This technique takes advantage of chromosome painting probes, which allow classification of all chromosomes. SKY can also identify complex chromosome aberrations and segregation defects in mice and humans with various diseases, including polycystic kidney disease. Biology Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA Ashwin Prakash1, Jason Bechtel1, Alexei Fedorov1 1Department of Medicine, University of Toledo Health Science Campus We present a public computational web site for the analysis of genomic sequences. It detects DNA sequence patterns with various non-random nucleotide compositions. This resource also generates randomized sequences with diverse levels of complexity.