University of Wisconsin, Milwaukee View Institution's Website 11 articles published in JoVE Bioengineering Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings Viviana Zlochiver*1, Stacie L. Kroboth*1, Christopher R. Beal1, Jonathan A. Cook1, Rosy Joshi-Mukherjee1,2,3 1Aurora Research Institute, Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science, University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine, Johns Hopkins University This article contains a set of protocols for the development of human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) networks cultured on multiwell MEA plates to reversibly electroporate the cell membrane for action potential measurements. High-throughput recordings are obtained from the same cell sites repeatedly over days. Engineering Force-Clamp Rheometry for Characterizing Protein-based Hydrogels Luai R. Khoury1, Joel Nowitzke1, Narayan Dahal1, Kirill Shmilovich1, Annie Eis1, Ionel Popa1 1Department of Physics, University of Wisconsin-Milwaukee A new force-clamp rheometry technique is used to investigate the mechanical properties of low-volume protein-based hydrogel samples tethered between a voice-coil motor and a force sensor. An analog proportional-integral-derivative (PID) system allows for the 'clamping' of the force experienced to the desired protocol. Engineering Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors Jiang Yang1,2, Tae-Joon Kwak3, Xiaodong Zhang4, Robert McClain5, Woo-Jin Chang*3,6, Sundaram Gunasekaran*1 1Department of Biological Systems Engineering, University of Wisconsin-Madison, 2Environment, Energy and Natural Resources Center, Department of Environmental Science and Engineering, Fudan University, 3Department of Mechanical Engineering, University of Wisconsin-Milwaukee, 4Department of Physics, School of Science, Tianjin University, 5Department of Chemistry, University of Wisconsin-Madison, 6School of Freshwater Sciences, University of Wisconsin-Milwaukee The study demonstrates the growth of iridium oxide-reduced graphene oxide (IrO2-RGO) nanohybrid thin films on irregular and rough screen-printed carbon substrate through a green electrochemical synthesis, and their implementation as a pH sensor with a patterned paper-fluidic platform. Immunology and Infection Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress Laura M. Ketelboeter1, Sonia L. Bardy1 1Department of Biological Sciences, University of Wisconsin Milwaukee Bacterial pyomelanin production results in increased resistance to oxidative stress and virulence. We report on techniques that can be used to determine inhibition of pyomelanin production and assay the resulting increase in sensitivity to oxidative stress in bacteria, as well as determine antibiotic minimum inhibitory concentration (MIC). Engineering Fabrication and Characterization of Disordered Polymer Optical Fibers for Transverse Anderson Localization of Light Salman Karbasi1, Ryan J. Frazier1, Craig R. Mirr1, Karl W. Koch2, Arash Mafi1 1Department of Electrical Engineering and Computer Science, University of Wisconsin-Milwaukee, 2Corning Incorporated, Corning, New York We develop and characterize a disordered polymer optical fiber that uses transverse Anderson localization as a novel waveguiding mechanism. This microstructured fiber can transport a small localized beam with a radius that is comparable to the beam radius of conventional optical fibers. Behavior How to Detect Amygdala Activity with Magnetoencephalography using Source Imaging Nicholas L. Balderston1, Douglas H. Schultz1, Sylvain Baillet2,3, Fred J. Helmstetter1,3 1Department of Psychology, University of Wisconsin-Milwaukee, 2McConnell Brain Imaging Centre, Montreal Neurological Institute, McGill University, 3Department of Neurology, Medical College of Wisconsin This article describes how to record amygdala activity with magnetoencephalography (MEG). In addition this article will describe how to conduct trace fear conditioning without awareness, a task that activates the amygdala. It will cover 3 topics: 1) Designing a trace conditioning paradigm using backward masking to manipulate awareness. 2) Recording brain activity during the task using magnetoencephalography. 3) Using source imaging to recover signal from subcortical structures. Neuroscience Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents Marie Vandecasteele1,2, S. M.1, Sébastien Royer1,3, Mariano Belluscio1, Antal Berényi1, Kamran Diba1,4, Shigeyoshi Fujisawa1, Andres Grosmark1, Dun Mao1, Kenji Mizuseki1, Jagdish Patel1, Eran Stark1, David Sullivan1, Brendon Watson1, György Buzsáki1 1Center for Molecular and Behavioral Neuroscience, University of New Jersey, 2Center for Interdisciplinary Research in Biology, Collège de France, 3Janelia Farm Research Campus, Howards Hughes Medical Institute, 4Deptartment of Psychology, University of Wisconsin at Milwaukee We describe methods for large-scale recording of multiple single units and local field potential in behaving rodents with silicon probes. Drive fabrication, probe attachment to the drive and probe implantation processes are illustrated in sufficient details for easy replication. Biology In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy Michael Stoneman1, Deo Singh1, Valerica Raicu1,2 1Department of Physics, University of Wisconsin - Milwaukee, 2Department of Biological Sciences, University of Wisconsin - Milwaukee By employing a spectrally resolved two-photon microscopy imaging system, pixel-level maps of Förster Resonance Energy Transfer (FRET) efficiencies are obtained for cells expressing membrane receptors hypothesized to form homo-oligomeric complexes. From the FRET efficiency maps, we are able to estimate stoichiometric information about the oligomer complex under study. Application Notes Feeder非存在下でのヒトiPS細胞の凍結保存 - ADVERTISEMENT Michael Stoneman1, Deo Singh1, Valerica Raicu1,2 1Department of Physics, University of Wisconsin - Milwaukee, 2Department of Biological Sciences, University of Wisconsin - Milwaukee Biology Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies Vinod B. Shidham1, George M. Varsegi1, Krista D'Amore1, Anjani Shidham2 1Department of Pathology, University of Wisconsin - Milwaukee, 2BioInnovation LLC Practicing of fine needle aspiration biopsies (FNAB) by trainees is relatively challenging, due to the lack of an easily available, appropriate lesion. Preparation of an AV phantom lesion for practicing the FNAB procedure and mastering proficiency is relatively easy. Biology Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells George M. Varsegi1, Vinod Shidham1 1Department of Pathology, University of Wisconsin - Milwaukee Shidham's method for preparation of cell blocks with AV-marker from cytology specimens containing individually scattered cells and small cell groups.
