Federal University of Sao Carlos (UFSCAR) 2 articles published in JoVE Biochemistry Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates Patrícia M. S. dos Passos*1, Valentine Spagnol*2, Camila R.S.T.B de Correia1, Felipe R. Teixeira1,2 1Department of Genetic and Evolution, Federal University of São Carlos, 2Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo We provide a detailed protocol for a ubiquitylation assay of a specific substrate and an E3 ubiquitin-ligase in mammalian cells. HEK293T cell lines were used for protein overexpression, the polyubiquitylated substrate was purified from cell lysates by immunoprecipitation, and resolved in SDS-PAGE. Immunoblotting was used to visualize this post-translational modification. Chemistry Niobium Oxide Films Deposited by Reactive Sputtering: Effect of Oxygen Flow Rate Silvia L. Fernandes1, Lucas J. Affonço2, Roberto A. R. Junior2, José H. D. da Silva2, Elson Longo1, Carlos F. de O. Graeff2 1Chemistry Department, Federal University of São Carlos (UFSCAR), 2Physics Department, School of Sciences, São Paulo State University (UNESP) Here, we present a protocol for niobium oxide films deposition by reactive sputtering with different oxygen flow rates for use as an electron transport layer in perovskite solar cells.
