Unidad Academica Bioquimica 1 article published in JoVE Medicine High-Resolution Respirometry to Assess Mitochondrial Function in Human Spermatozoa Pilar Irigoyen1, Rossana Sapiro1,3, Adriana Cassina2,3 1Departamento de Histología y Embriología, Unidad Académica Histología y Embriología, 2Departamento de Bioquímica, Unidad Académica Bioquímica, 3Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina, Universidad de la República The analysis of sperm mitochondrial function by high-resolution respirometry permits the measurement of the oxygen consumption of freely moving spermatozoa in a closed-chamber system. The technique can be applied to measure respiration in human spermatozoa, which provides information on sperm mitochondrial characteristics and integrity.
