JoVE   
You have subscription access to articles in this section through JoVE.

  JoVE Biology

  
You have subscription access to articles in this section through JoVE.

  JoVE Neuroscience

  
You have subscription access to articles in this section through JoVE.

  JoVE Immunology and Infection

  
You have subscription access to articles in this section through JoVE.

  JoVE Clinical and Translational Medicine

  
You have subscription access to articles in this section through JoVE.

  JoVE Bioengineering

  
You have subscription access to articles in this section through JoVE.

  JoVE Applied Physics

  
You have subscription access to articles in this section through JoVE.

  JoVE Chemistry

  
You have subscription access to articles in this section through JoVE.

  JoVE Behavior

  
You have subscription access to articles in this section through JoVE.

  JoVE Environment

|   

JoVE Science Education

General Laboratory Techniques

You have subscription access to videos in this collection through JoVE.

Basic Methods in Cellular and Molecular Biology

You have subscription access to videos in this collection through JoVE.

Model Organisms I

You have subscription access to videos in this collection through JoVE.

Model Organisms II

You have trial access to videos in this collection until May 31, 2014.

Refine your search:

Containing Text
Filter by author or institution
GO
Filter by publication date
From:
October, 2006
Until:
Today
Filter by section
Biology
Neuroscience
Immunology and Infection
Clinical and Translational Medicine
Bioengineering
Applied Physics
Chemistry
Behavior
Environment
 
 
Electrophoresis, Gel, Two-Dimensional: Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
 JoVE Immunology and Infection

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)

1Department of Microbiology, Immunology and Pathology, Colorado State University


JoVE 3104

Leprosy, caused by Mycobacterium leprae, is still endemic in many places. In order to learn about the spread and mode of transmission of leprosy, it is important to determine which strain of M. leprae has infected a patient. Variable numbers of tandem repeats (VNTR) typing is one such method.

 JoVE Biology

Pouring and Running a Protein Gel by reusing Commercial Cassettes

1Department of Biology, University of Florida, 2UF Genetics Institute, University of Florida, 3Plant Molecular & Cellular Biology Program, University of Florida


JoVE 3465

Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.

 JoVE Biology

DNA Methylation: Bisulphite Modification and Analysis

1Epigenetics Group, Cancer Research Program, Garvan Institute of Medical Research, 2St Vincent's Clinical School, University of NSW


JoVE 3170

The gold standard for DNA methylation analysis is genomic sequencing of bisulphite converted DNA. This method takes advantage of the increased sensitivity of cytosine compared with 5-methylcytosine (5-MeC) to bisulphite deamination under acidic conditions. Unmethylated cytosines can be distinguished from methylated cytosines after PCR amplification of the target genomic DNA.

 JoVE Bioengineering

Preparation of Complaint Matrices for Quantifying Cellular Contraction

1Institute for Biophysical Dynamics, University of Chicago, 2Physics Department - James Franck Institute, University of Chicago, 3Interdisciplinary Scientist Training Program, University of Chicago


JoVE 2173

In this video, we demonstrate the experimental techniques used to fabricate compliant, extracellular matrix (ECM) coated substrates suitable for cell culture, and which are amenable to traction force microscopy and observing effects of ECM stiffness on cell behavior.

 JoVE Biology

FtsZ Polymerization Assays: Simple Protocols and Considerations

1Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen


JoVE 50844

Polymerization of FtsZ is essential for bacterial cell division. In this report, we detail simple protocols to monitor FtsZ polymerization activity and discuss the influence of buffer composition. The protocols can be used to study the interaction of FtsZ with regulatory proteins or antibacterial drugs that affect FtsZ polymerization.

 JoVE Biology

Methods for the Study of the Zebrafish Maxillary Barbel

1Department of Biological Sciences, DePaul University, 2Children’s Memorial Research Center, Department of Pediatrics, Northwestern University Feinberg School of Medicine


JoVE 1558

The zebrafish maxillary barbel is an integumentary sense organ containing ectodermal, mesodermal and neural crest derivatives. Importantly, the adult barbel can regenerate after proximal amputation. This video introduces maxillary barbel development and demonstrates a surgical protocol to induce regeneration, followed by collection, embedding and downstream imaging of barbel specimens.

 JoVE Biology

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

1Department of Experimental Oncology, European Institute of Oncology


JoVE 51220

By combining native and crosslinking chromatin immunoprecipitation with high-resolution Mass Spectrometry, ChroP approach enables to dissect the composite proteomic architecture of histone modifications, variants and non-histonic proteins synergizing at functionally distinct chromatin domains.

 JoVE Biology

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

1Pall Life Sciences


JoVE 2407

This protocol is a cost effective alternative for efficient parallel clarification and plasmid DNA purification from E. coli cultures. The AcroPrep Advance process starts with an optimized lysate clarification filter plate followed by purification on a high binding capacity DNA binding filter plate.

 JoVE Biology

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

1Institute of Plant Biology and Biotechnology, University of Münster, 2Department of Plant Biology, Carnegie Institution for Science


JoVE 51103

The described comparative, quantitative proteomic approach aims at obtaining insights into the composition of multiprotein complexes under different conditions and is demonstrated by comparing genetically different strains. For quantitative analysis equal volumes of different fractions from a sucrose density gradient are mixed and analyzed by mass spectrometry.

 JoVE Biology

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

1Protein Expression and Purification Core Facility, EMBL Heidelberg


JoVE 1350

A short protocol for protein staining with Coomassie Brilliant Blue (CBB) G-250 in polyacrylamide gels is described without using organic solvents or acetic acid as in the classical staining procedures with CBB.

More Results...
Waiting
simple hit counter