The recorded electrical responses from nerve, muscle, Sensory receptor
, or area of the Central nervous system
following stimulation. They range from less than a microvolt to several microvolts. The evoked potential can be auditory (Evoked potentials
), somatosensory (Evoked potentials
), visual (Evoked potentials
), or motor (Evoked potentials
), or other modalities that have been reported. Often used synonymously to event-related potentials which are associated with higher level cognitive processes.
JoVE Immunology and Infection
1INSERM UMR 1043, CNRS UMR 5282, Université Toulouse 3, 2UMR Viroligie, INRA ENVA ANSES
These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.
1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), University of Heidelberg
Protein conformation and dynamics are key to understanding the relationship between protein structure and function. Hydrogen exchange coupled with high-resolution mass spectrometry is a versatile method to study the conformational dynamics of proteins as well as characterizing protein-ligand and protein-protein interactions, including contact interfaces and allosteric effects.
1Wolfson Centre for Age-Related Diseases, King's College London, 2David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
Neuroblast migration is a fundamental event in postnatal neurogenesis. We describe a protocol for efficient labeling of neuroblasts by in vivo postnatal electroporation and subsequent visualization of their migration using time-lapse imaging of acute brain slices. We include a description for the quantitative analysis of neuroblast dynamics by video tracking.
1Department of Biology, University of Kentucky, 2Department of Biology, University of Salahaddin, 3Oregon Institute of Marine Biology, University of Oregon
This article describes the cockroach ventral nerve cord dissection and extracellular recordings from the cercal nerve and connectives. Evoked responses are generated by electrical stimulation of the cercal nerve or direct mechanical stimulation of the cerci.
1Departments of Pediatrics and Neurology, University of Michigan
Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.
1Neurobiology Section, Division of Biological Sciences, University of California-San Diego, 2Department of Neuroscience, Columbia University, 3Dart NeuroScience, 4School of Dental Medicine, University of Pennsylvania
In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster.
1Laboratory of Neural Microcircuitry - Brain Mind Institute, Ecole Polytechnique Federale de Lausanne
Multi-electrode patch-clamp recordings constitute a complex task. Here we show how, by automating of many of the experimental steps, it is possible to accelerate the process leading to qualitative improvement in performance and number of recordings.
1Department of Biological Sciences, University of Alberta
We have developed an intact brain-spinal cord preparation to record and monitor electrical activity via patch clamp recording from the Mauthner neurons and other reticulospinal cells in zebrafish embryos. Thus, we are able to record excitatory and inhibitory synaptic currents, voltage-gated channel activity and action potentials from key neurons in a developing embryo.
1Department of Biology, University of Kentucky, 2Center of Muscle Biology, University of Kentucky, 3Oregon Institute of Marine Biology, University of Oregon
Physiological and anatomical techniques are demonstrated to address function and structure for joint proprioceptors and muscle tension receptors in crustacean walking limbs.
1Endocrine Research Laboratory and Department of Medicine, Royal Victoria Hospital, 2McGill University Health Centre Research Institute
The zebrafish, a small tropical fish, has become a popular model for studying gene function during vertebrate development and disease. The temporal and spatial expression of target genes can be determined by in situ hybridization. Our improved protocol allows for the detection of low abundant transcripts with low non-specific background signal.