Submit
Browse All

The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

Recommend to Librarian

Refine your search:

Containing Text
Filter by author or institution
GO
Filter by publication date
From:
October, 2006 Until:
Today
Filter by section
General
Neuroscience
Immunology and Infection
Clinical and Translational Medicine
Bioengineering
Applied Physics
Chemistry
 
 
 JoVE Clinical and Translational Medicine

Portable Intermodal Preferential Looking (IPL): Investigating Language Comprehension in Typically Developing Toddlers and Young Children with Autism


JoVE 4331 12/14/2012

,

Department of Psychology, University of Connecticut

A reliable home-based way to assess the language comprehension of very young typically developing children, as well as those with autism, is described. The method analyzes children's eye gaze while viewing side-by-side images but hearing an audio that matches only one image. Stimuli are designed with young participants in mind.

Results below contain some, but not all of your search terms.
 JoVE Neuroscience

C. elegans Tracking and Behavioral Measurement


JoVE 4094 11/17/2012

1, 2,3, 1, 1,4

1Donnelly Centre, University of Toronto, 2Department of Physics and Astronomy, Vrije Universiteit, 3Okinawa Institute of Science and Technology, 4Department of Physics, University of Toronto

We have developed a video-rate tracking microscope system that can record and quantify C. elegans behavior at high resolution and high speeds. We have also developed computational methods to reduce the dimensionality of the worm images to a fundamental set of measurements that completely describe the shape of the worm.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE Applied Physics

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water


JoVE 3975 8/02/2012

1, 2, 3, 3, 2

1Organic Chemistry Institute and CeNTech, Westfälische Wilhelms-Universität Münster, 2Laboratory of Macromolecular and Organic Chemistry, Institute for Complex Molecular Systems, Eindhoven University of Technology, 3Laboratory of Materials and Interface Chemistry and Soft Matter Research Unit, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology

The goal of this experiment is to determine and control the size, shape and stability of self-assembled discotic amphiphiles in water. For aqueous based supramolecular polymers such level of control is very difficult. We apply a strategy using both repulsive and attractive interactions. The experimental techniques applied to characterize this system are broadly applicable.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE General

Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration


JoVE 2845 8/29/2011

*, *,

Department of Biological Sciences, University of Notre Dame

Acute kidney injury (AKI) in humans is a common clinical problem caused by damage to the epithelial cells that comprise kidney nephrons, and AKI is associated with high mortality rates of 50-70%1. Following epithelial cell destruction, nephrons have a limited ability to regenerate, though the mechanisms and limitations that guide this phenomenon remain poorly understood. In this video article, we describe our technique for targeted laser ablation of kidney nephron cells in the zebrafish embryo kidney, or pronephros. Our new method can be used to complement nephrotoxicity-induced models of AKI and gain a high-resolution understanding of the cell and molecular alterations that are associated with epithelial regeneration in the kidney nephron.

Results below contain some, but not all of your search terms.
 JoVE Neuroscience

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures


JoVE 50273 4/03/2013

1, 1, 2, 1

1Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 2Department of Microbiology and Molecular Genetics, Medical College of Wisconsin

The localization and distribution of proteins provide important information for understanding their cellular functions. The superior spatial resolution of electron microscopy (EM) can be used to determine the subcellular localization of a given antigen following immunohistochemistry. For tissues of the central nervous system (CNS), preserving structural integrity while maintaining antigenicity has been especially difficult in EM studies. Here, we adopt a procedure that has been used to preserve structures and antigens in the CNS to study and characterize synaptic proteins in rat hippocampal CA1 pyramidal neurons.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE General

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila


JoVE 1510 8/20/2009

1, 2, 3, 3, 2, 1

1Center for Systems Biology, Massachusetts General Hospital, 2Institute for Biological and Medical Imaging (IBMI), Technical University of Munich and Helmholtz Center Munich, 3Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute

Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE Clinical and Translational Medicine

Dorsal Column Steerability with Dual Parallel Leads using Dedicated Power Sources: A Computational Model


JoVE 2443 2/10/2011

, ,

Boston Scientific , Neuromodulation

Using a mathematical model of spinal cord stimulation, we found that a multi-source system with independent power sources for each contact can target more central points of stimulation on the dorsal column (100 vs 3) and has 50-fold more field steering resolution (0.02mm vs 1mm) than a single-source system.

Results below contain some, but not all of your search terms.
 JoVE Bioengineering

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers


JoVE 3405 10/13/2011

1, 2, 2

1LSA Biophysics, University of Michigan, 2LSA Biophysics, Department of Physics, University of Michigan

We illustrate the use of a constant force axial optical tweezers to explore the mechanical properties of short DNA molecules. By stretching DNA axially, we minimize steric hindrances and artifacts arising in conventional lateral manipulation, allowing us to study DNA molecules as short as ~100 nm.

Results below contain some, but not all of your search terms.
 JoVE General

Associated Chromosome Trap for Identifying Long-range DNA Interactions


JoVE 2621 4/23/2011

,

Medical Service, VA Palo Alto Health Care System , Stanford University School of Medicine

The associated chromosome trap (ACT) assay is a novel unbiased method for identifying long-range DNA interactions. The characterization of long range DNA interactions will allow us to determine the relationship of nuclear architecture to gene expression in both normal physiology and in diseased states.

Results below contain some, but not all of your search terms.
 JoVE Immunology and Infection

Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure


JoVE 3221 8/23/2011

, , ,

Vascular Pharmacology Laboratory, Baker IDI Heart and Diabetes Institute, Monash University

This is a method to visualise leukocyte adhesion to the endothelium in harvested pressurised vessels. The technique enables studying vascular adhesion under shear flow with differing intraluminal pressures up to 200 mmHg thus mimic-ing the pathophysiological conditions of high blood pressure.

Results below contain some, but not all of your search terms.
 JoVE General

Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation


JoVE 3770 4/30/2012

*1, *1,2,3, 1, 1, 1, 1, 1, 1,3

1Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, 2A*STAR-Duke-NUS Neuroscience Research Partnership, Singapore, 3Department of Biochemistry, National University of Singapore, Singapore

Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET) is a method for de novo detection of chromatin interactions, for better understanding of transcriptional control.

Results below contain some, but not all of your search terms.
 JoVE Immunology and Infection

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells


JoVE 4028 6/03/2012

,

Department of Virology, Universitätsklinikum Freiburg

Influenza viruses replicate their RNA genome in association with host-cell chromatin. Here, we present a method to purify intact viral ribonucleoprotein complexes from the chromatin of infected cells. Purified viral complexes can be analyzed by both Western blot and primer extension of protein and RNA content, respectively.

Results below contain some, but not all of your search terms.
 JoVE General

Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice


JoVE 404 10/31/2007

, ,

Diabetes Center, University of California, San Francisco - UCSF

Our protocol was developed to cleanly and easily deliver islets or cells under the kidney capsule of mice. Cells are concentrated into pellets in the final tubing used for transplanting the cells under the kidney capsule. The ease of this technique reduces stress to the cells and the mouse.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE Editorial

JoVE 3rd Issue


JoVE 178 5/01/2007

This third issue of JoVE draws attention to issues on the intersection of the basic and applied biomedical research. In this context, the interview with Ole Isacson (McLean Hospital/Harvard Medical School) provides an in-depth look at contemporary challenges of Parkinson’s disease research. The candid interview grants insights that reach beyond the pure scientific problems, as it addresses...

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE General

Analyzing and Building Nucleic Acid Structures with 3DNA


JoVE 4401 4/26/2013

1, 2, 1

1Department of Chemistry & Chemical Biology and BioMaPS Institute for Quantitative Biology, Rutgers - The State University of New Jersey, 2Department of Biological Sciences, Columbia University

The 3DNA software package is a popular and versatile bioinformatics tool with capabilities to analyze, construct, and visualize three-dimensional nucleic acid structures. This article presents detailed protocols for a subset of new and popular features available in 3DNA, applicable to both individual structures and ensembles of related structures.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE Neuroscience

VisualEyes: A Modular Software System for Oculomotor Experimentation


JoVE 2530 3/25/2011

, ,

Department of Biomedical Engineering, New Jersey Institute of Technology

Neural control and cognitive processes can be studied through eye movements. The VisualEyes software allows an operator to program stimuli on two computer screens independently using a simple, custom scripting language. The system can stimulate tandem eye movements (saccades and smooth pursuit) or opposing eye movements (vergence) or any combination.

Results below contain some, but not all of your search terms.
 JoVE Clinical and Translational Medicine

Creation of Reversible Cholestatic Rat Model


JoVE 2692 5/21/2011

, *, *, *

Department of General Surgery, Providence Hospital and Medical Centers

Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Creation of a reversible cholestatic rat model can be challenging in view of the smaller size and unique hepatopancreatobiliary anatomy in rats. This video article demonstrates the creation of a reversible cholestatic model.

Results below contain some, but not all of your search terms.
 JoVE General

Imaging In-Stent Restenosis: An Inexpensive, Reliable, and Rapid Preclinical Model


JoVE 1346 9/14/2009

1, 2, 1, 1

1Department of Cardiothoracic Surgery, Stanford University School of Medicine, 2Stanford University School of Medicine

This video demonstrates how to use a preclinical inexpensive and reliable model to study pathobiological and pathophysiological processes of in-stent restenosis development. Longitudinal in vivo monitoring using OCT (Optical Coherence Tomography) and analysis of OCT images are also demonstrated.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE General

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels


JoVE 3850 4/24/2012

, ,

Department of Pharmacology, Physiology & Neuroscience, University of South Carolina, School of Medicine

A real-time screening procedure for identifying drugs that interact with G protein-gated inward rectifier K+ (GIRK) channels is described. The assay utilizes membrane potential-sensitive fluorescent dyes to measure GIRK channel activity. This technique is adaptable for use on a number of cell lines.

Results below contain some, but not all of your search terms.
 JoVE General

Dissection of Saccharomyces Cerevisiae Asci


JoVE 1146 5/19/2009

, ,

Department of Biology, Concordia University

Micromanipulation of yeast cells is needed for meiotic genetic analysis or to select diploid zygotes. These micromanipulations are carried out using the microneedle of a dissection microscope. The microneedle is used to relocate cells and is controlled by a micromanipulator which are available with various degrees of automation.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE Clinical and Translational Medicine

An Investigation of the Effects of Sports-related Concussion in Youth Using Functional Magnetic Resonance Imaging and the Head Impact Telemetry System


JoVE 2226 1/12/2011

1,2,3,4,5, 1, 1, 1, 3, 6,7

1Graduate Department of Rehabilitation Science, University of Toronto, 2Occupational Science and Occupational Therapy, University of Toronto, 3Department of Psychology, University of Toronto, 4Bloorview Kids Rehab, 5Toronto Rehab, 6Cognitive Neurology, Sunnybrook Health Sciences Centre, 7Faculty of Medicine, University of Toronto

This article provides an overview of a multi-modal approach to mild traumatic brain injury diagnosis and recovery in youth. This approach combines neuropsychological testing with functional magnetic resonance imaging and the Head Impact Telemetry System to monitor the relationship between head impacts and brain activity during cognitive testing.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE Neuroscience

Correlating Behavioral Responses to fMRI Signals from Human Prefrontal Cortex: Examining Cognitive Processes Using Task Analysis


JoVE 3237 6/20/2012

1,2, 1, 1

1Department of Psychology, Centre for Vision Research, York University, 2Department of Biology, Centre for Vision Research, York University

The goal of our research is to correlate behavior to brain activity. Accurate behavioral measures and imaging techniques allow us to elucidate brain-behavior relationships.

Results below contain some, but not all of your search terms.
 JoVE Clinical and Translational Medicine

Stereotactic Radiosurgery for Gynecologic Cancer


JoVE 3793 4/17/2012

1, 1, 2

1Department of Radiation Oncology, University Hospitals Case Medical Center and Case Western Reserve University School of Medicine, 2Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, University Hospitals Case Medical Center and Case Western Reserve University School of Medicine

Stereotactic body radiotherapy (SBRT) involves image-guided, ablative radiation delivered to cancer targets refractory to chemotherapy or to conventional radiation treatment. The robotic-armed Cyberknife SBRT system, using sophisticated target localization, delivers hypofractionated radiation doses capable of sterilizing cancer targets. This article will consider new therapeutic roles of SBRT for gynecological cancers.

Results below contain some, but not all of your search terms.
 JoVE Clinical and Translational Medicine

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model


JoVE 50086 11/13/2012

1, 2,3, 1

1Program in Molecular Medicine and Department of Cancer Biology, University of Massachusetts Medical School, 2Departments of Surgery and Medicine, Weill Cornell Medical College, 3Departments of Surgery and Medicine, New York Presbyterian Hospital

A rapid way to screen for melanoma modifiers using a zebrafish autochthonous tumor model is presented. It takes advantage of the miniCoopR vector which allows for expression of candidate melanoma genes in melanocytes. A method to obtain melanoma-free survival curves, an invasion assay, a protocol for antibody staining of scale melanocytes and a melanoma transplantation assay are described.

Results below contain some, but not all of your search terms.
 JoVE Bioengineering

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels


JoVE 50081 12/06/2012

,

Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University - Purdue University at Indianapolis

The following protocol provides techniques for encapsulating pancreatic β-cells in step-growth PEG-peptide hydrogels formed by thiol-ene photo-click reactions. This material platform not only offers a cytocompatible microenvironment for cell encapsulation, but also permits user-controlled rapid recovery of cell structures formed within the hydrogels.

Results below contain some, but not all of your search terms.
 JoVE General

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells


JoVE 4254 10/11/2012

, ,

Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California

We present a simple protocol to visualize regions of programmed cell death (PCD) in mouse embryos and differentiating embryonic stem (ES) cell cultures using a highly soluble dye called LysoTracker.

Results below contain some, but not all of your search terms.
 JoVE General

Use of Arabidopsis eceriferum Mutants to Explore Plant Cuticle Biosynthesis


JoVE 709 5/31/2008

1, 1, 1, 1, 1,2, 1

1Department of Botany, University of British Columbia - UBC, 2Department of Chemistry, University of British Columbia - UBC

The plant cuticle is a waxy outer covering on plants that has a primary role in water conservation but is also an important barrier against the entry of pathogenic microorganisms. In this video, we demonstrate the analysis of plant cuticle mutants identified by forward and reverse genetics approaches.

Results below contain some, but not all of your search terms.
 JoVE Neuroscience

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila


JoVE 50306 3/28/2013

,

Neurodevelopment Group, School of Biosciences, University of Birmingham

An injury paradigm using the Drosophila larval ventral nerve cord to investigate central nervous system regeneration and repair is described. Stabbing followed by laser scanning confocal microscopy in time-lapse and fixed specimens, combined with quantitative analysis with purposefully developed software and genetics, are used to investigate the molecular mechanisms of CNS regeneration and repair.

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
 JoVE General

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.


JoVE 1869 5/06/2010

*1, *2,3,4,5, *2, 6, 2, 3,6, 1, 2,7,8

1Program in Gene Function and Expression, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 2Broad Institute of Harvard and Massachusetts Institute of Technology, 3Division of Health Sciences and Technology, Massachusetts Institute of Technology, 4Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics, Harvard University, 5Department of Applied Mathematics, Harvard University, 6Department of Physics, Massachusetts Institute of Technology, 7Department of Systems Biology, Harvard Medical School, 8Department of Biology, Massachusetts Institute of Technology

The Hi-C method allows unbiased, genome-wide identification of chromatin interactions (1). Hi-C couples proximity ligation and massively parallel sequencing. The resulting data can be used to study genomic architecture at multiple scales: initial results identified features such as chromosome territories, segregation of open and closed chromatin, and chromatin structure at the megabase scale.

Results below contain some, but not all of your search terms.
 JoVE General

In-vivo Centrifugation of Drosophila Embryos


JoVE 2005 6/23/2010

,

Department of Biology, University of Rochester

We describe a method to separate organelles by density in living Drosophila embryos. Embryos are embedded in agar and centrifuged. This technique yields reproducible separation of major organelles along the anterior-posterior embryo axis. This method facilitates colocalization experiments and yields organelle fractions for biochemical analysis and transplantation experiments.

Results below contain some, but not all of your search terms.
 JoVE Immunology and Infection

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells


JoVE 2186 10/06/2010

1, 2, 1, 2

1Department of Physiology, Dartmouth College, 2Department of Biology, Indiana University Purdue University Indianapolis

This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.

Results below contain some, but not all of your search terms.
 JoVE Bioengineering

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)


JoVE 2664 6/08/2011

, ,

School of Engineering, University of Vermont

Secondary electrospray ionization mass spectrometry (SESI-MS) enables the detection of volatile organic compounds (VOCs) without the need for any sample pretreatment. This protocol provides instructions for the rapid (within minutes) characterization of bacterial VOCs using SESI-MS.

More Results...
Waiting
simple hit counter