Polymer Microarrays for High Throughput Discovery of Biomaterials

JoVE 3636 1/25/2012

Andrew L. Hook¹, Chien-Yi Chang², Jing Yang¹, David J. Scurr¹, Robert Langer³, Daniel G. Anderson³, Steve Atkinson², Paul Williams², Martyn C. Davies¹, Morgan R. Alexander¹

¹Laboratory of Biophysics and Surface Analysis, University of Nottingham , ²School of Molecular Medical Sciences, University of Nottingham , ³David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology

A description of the formation of a polymer microarray using an on-chip photopolymerization technique. The high throughput surface characterization using atomic force microscopy, water contact angle measurements, X-ray photoelectron spectroscopy and time of flight secondary ion mass spectrometry and a cell attachment assay is also described.

Comprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates

JoVE 1837 3/12/2010

Cliff E. Foster¹, Tina M. Martin¹, Markus Pauly²

¹Great Lakes Bioenergy Research Center, Michigan State University (MSU), ²Great Lakes Bioenergy Research Center and DOE-Plant Research Lab, Michigan State University (MSU)

Plant biomass is a major carbon-neutral renewable resource that could be used for the production of biofuels. Plant biomass consists mainly of cell walls, a structurally complex composite material termed lignocellulosics. Here we describe a protocol for a comprehensive analysis of the content and composition of wall derived carbohydrates.

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

JoVE 3314 10/24/2011

Jonathan Walton^1,2, Goutami Banerjee², Suzana Car²

¹DOE Plant Research Laboratory, Michigan State University, ²DOE Great Lakes Bioenergy Research Center, Michigan State University

GENPLAT (GLBRC Enzyme Platform) is an automated platform for discovery and optimization of enzyme cocktails for biomass degradation. It can be adapted to multiple feedstocks and mixtures of enzymes containing multiple components.

Obtaining Hemocytes from the Hawaiian Bobtail Squid Euprymna scolopes and Observing their Adherence to Symbiotic and Non-Symbiotic Bacteria

JoVE 1714 2/11/2010

Andrew J. Collins, Spencer V. Nyholm

Department of Molecular and Cell Biology, University of Connecticut

This video will demonstrate how to obtain hemocytes (blood cells) from the Hawaiian bobtail squid, Euprymna scolopes for use in cell biological and bacterial adhesion assays. Hemocytes will be stained with a fluorescent dye and exposed to GFP-labeled bacteria.

Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium

JoVE 2563 2/27/2011

Alison Claybon, Alexander J. R. Bishop

UT Health Science Center at San Antonio, Greehey Children's Cancer Research Institute and Department of Cellular and Structural Biology

A formal demonstration of the dissection of a mouse eye, resulting in a whole mount of the retinal pigment epithelium.

An Interview with Nobel Laureate Douglas Osheroff, Physics 1996

JoVE 1540 7/07/2009

Douglas Osheroff

At extremely low temperatures, the molecules of certain fluids are able to flow without internal friction or resistance - a phenomenon know as superfluidity. In this interview, Nobel Laureate Douglas Osheroff (Physics 1996) describes the somewhat arduous quest to prove the predicted superfluid state of helium-3. He also shares his thoughts on mentoring undergraduates and his philosophy on getting the most out of graduate school.

Aseptic Laboratory Techniques: Plating Methods

JoVE 3064 5/11/2012

Erin R. Sanders

Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles

When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.

The Waters Xevo™ TQ-S Equipped With New StepWave™ and ScanWave™ Technology - ADVERTISEMENT

JoVE 2268 6/15/2010

Robert S. Plumb

Pharmaceutical Business Operations, Waters Corporation

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

JoVE 2763 3/26/2011

Larissa A. Pikor*^1,2, Katey S. S. Enfield*^1,2, Heryet Cameron³, Wan L. Lam^1,2,4

¹Department of Integrative Oncology, BC Cancer Research Centre, ²Interdisciplinary Oncology Program, University of British Columbia - UBC, ³Photography/Video Production, Multi-Media Services, BC Cancer Agency, ⁴Department of Pathology and Laboratory Medicine, University of British Columbia - UBC

This video demonstrates the protocol for DNA extraction from formalin-fixed paraffin-embedded material. This is a multi-day procedure in which tissue sections are deparaffinized with xylene, rehydrated with ethanol and treated with proteinase K to purify and isolate DNA for subsequent gene-specific or genome-wide analysis.

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

JoVE 2889 6/09/2011

Philip M. Armstrong, Theodore G. Andreadis, Shannon L. Finan, John J. Shepard, Michael C. Thomas

Center for Vector Biology and Zoonotic Diseases, Department of Environmental Sciences, The Connecticut Agricultural Experiment Station

We describe a method to process and screen field-collected mosquitoes for a diversity of viruses by Vero cell culture assay. By employing this technique, we have detected 9 different viruses from 4 taxonomic families in mosquitoes collected in Connecticut.