1Buck Institute for Research on Aging, 2Department of Physiology & Biophysics, University of Washington
Single cell expression profiling allows the detailed gene expression analysis of individual cells. We describe methods for the isolation of cardiomyocytes, and preparing the resulting lysates for either whole transcriptome microarray or qPCR of specific targets.
Published December 28, 2011. Keywords: Molecular Biology, Single cell analysis, Microarray, Gene expression, Cardiomyocyte, Mouse heart perfusion, mice, qPCR