Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice

JoVE 3756 2/23/2012

Victoria P. Le¹, Attila Kovacs², Jessica E. Wagenseil¹

¹Department of Biomedical Engineering, Saint Louis University, ²Department of Internal Medicine, Washington University School of Medicine

Measuring left ventricular pressure (LV) in embryonic and neonatal mice is described. Pressure is measured by inserting a needle connected to a fluid-filled transducer into the LV under ultrasound guidance. Care must be taken to maintain normal cardiac function during the experimental protocol.

A Swine Model of Neonatal Asphyxia

JoVE 3166 10/11/2011

Po-Yin Cheung¹, Richdeep S. Gill², David L. Bigam²

¹Departments of Pediatrics, Pharmacology and Surgery, University of Alberta, ²Department of Surgery, University of Alberta

Large animal models have good translational values in the examination of physiology and pharmacology of neonatal asphyxia. Using newborn piglets, we develop an experimental protocol to simulate neonatal asphyxia which has advantages of studying the systemic and regional hemodynamics, oxygen transport with biochemical and pathologic pathways and correlations.

Preterm EEG: A Multimodal Neurophysiological Protocol

JoVE 3774 2/18/2012

Susanna Stjerna¹, Juha Voipio², Marjo Metsäranta³, Kai Kaila^2,4, Sampsa Vanhatalo¹

¹Department of Children's Clinical Neurophysiology, Helsinki University Hospital, University of Helsinki , ²Department of Biosciences, University of Helsinki , ³Department of Pediatrics, Helsinki University Hospital, University of Helsinki , ⁴Neuroscience Center, University of Helsinki

This video explains the background theory of the neonatal EEG activity and the sensory responses, followed by a live demonstration of their recording in neonatal intensive care unit.

Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry

JoVE 3432 12/23/2011

Taha A. Jan, Renjie Chai, Zahra N. Sayyid, Alan G. Cheng

Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine

Flow cytometry is a powerful tool allowing for the isolation and study of specific cell populations. This protocol describes steps for isolating LacZ-expressing cells from cochlear tissues from neonatal transgenic mice. Dissociated cochlear cells were labeled using fluorescent-conjugated substrates of β-galactosidase prior to separation via flow cytometry.

In vivo Electroporation of Developing Mouse Retina

JoVE 2847 6/24/2011

Jimmy de Melo¹, Seth Blackshaw^1,2,3,4,5

¹Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, ²Department of Neurology, Johns Hopkins School of Medicine, ³Department of Ophthalmology, Johns Hopkins School of Medicine, ⁴Center for High-Throughput Biology, Johns Hopkins School of Medicine, ⁵Institute for Cell Engineering, Johns Hopkins School of Medicine

A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.

February 2012: This Month in JoVE

JoVE 4258 2/01/2012

Aaron Kolski-Andreaco

Delivery of Therapeutic Agents Through Intracerebroventricular (ICV) and Intravenous (IV) Injection in Mice

JoVE 2968 10/03/2011

Jacqueline J. Glascock¹, Erkan Y. Osman¹, Tristan H. Coady², Ferrill F. Rose¹, Monir Shababi³, Christian L. Lorson³

¹Department of Molecular Microbiology and Immunology, Bond Life Sciences Center, University of Missouri, ²Department of Biological Sciences, Columbia University , ³Department of Veterinary Pathobiology, Bond Life Sciences Center, University of Missouri

This article demonstrates two very different methods of injection: 1) into the brain (intracerebroventricular) and 2) systemic (intravenous) to introduce the therapeutic agents into the central nervous system of neonatal mice.

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

JoVE 652 1/25/2008

Ena Ladi, Paul Herzmark, Ellen Robey

Department of Molecular and Cell Biology, University of California, Berkeley

We present step-by-step instructions for the generation of neonatal chimeras as well as the dissection and preparation of the thymus for ex vivo imaging by 2-Photon Microscopy.

Measuring Fast Calcium Fluxes in Cardiomyocytes

JoVE 3505 11/29/2011

Urszula Golebiewska¹, Suzanne Scarlata²

¹Department of Biological Sciences and Geology, Queensborough Community College, ²Department of Physiology & Biophysics, Stony Brook University

We present a method to isolate rapid (microsecond) calcium events from slower fluxes in living cells using laser scanning confocal microscopy. The method measures fluorescence intensity fluctuations of calcium indicators by recording line scans of several hundred pixels in a cell. Histogram analysis allows us to isolate the time scales of different calcium fluxes.

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues

JoVE 3324 8/21/2011

Ryan W. O'Meara^1,2, Scott D. Ryan¹, Holly Colognato³, Rashmi Kothary^1,2,4

¹Regenerative Medicine Program, Ottawa Hospital Research Institute, ²Department of Cellular and Molecular Medicine, University of Ottawa , ³Department of Pharmacological Sciences, Stony Brook University, ⁴Department of Medicine, University of Ottawa

This article describes methods to derive enriched populations of murine oligodendrocyte precursor cells (OPCs) in primary culture, which differentiate to produce mature oligodendrocytes (OLs). In addition, this report describes techniques to produce murine myelinating co-cultures by seeding mouse OPCs onto a neurite bed of mouse dorsal root ganglion neurons (DRGNs).