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Chapter 7
Essential Cellular Processes

The Central Dogma
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins. RNA is the Missing Link…
Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity…
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform…
Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence.…
Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it…
Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation.…
What is the Cell Cycle?
The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two…
Interphase
The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of…
Mitosis and Cytokinesis
In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome…
The Cell Cycle Control System
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes…
Molecular Factors Affecting Cell Division
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the…
What is Meiosis?
Meiosis is the process by which diploid cells divide to produce haploid daughter cells. In humans, each diploid cell contains 46 chromosomes, half…
Overview of Cell Signaling
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes.…
Types of Signaling Molecules
In multicellular organisms, many molecules transmit signals between cells to pass information. These signals vary in complexity and include small…
G Protein-coupled Receptors
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate…
Cellular Differentiation
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array…
Use of <em>Drosophila</em> S2 Cells for Live Imaging of Cell Division
Drosophila S2 cells are an important tool in studying mitosis in tissue culture, providing molecular insights into this fundamental cellular process…
Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
Mitosis is critical for organismal growth and differentiation. The process is highly dynamic and requires ordered events to accomplish proper…
<em>In Vitro</em> Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
The placenta is the first organ to develop during embryogenesis and is required for the survival of the developing embryo. The placenta is comprised…

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