Overview
In this video, we demonstrate a staining procedure for cartilage using safranin-orange. This method helps visualize the cartilage and detect any damage.
Protocol
1. Safranin O staining
- Begin with the glass slides containing serial sagittal sections spanning a region from the center of the lateral condyle to the center of the medial condyle of a euthanized mouse model with the destabilized medial meniscus.
- Next, deparaffinize the slides in oxylene, and hydrate them in an ethanol gradient to distilled water. Thereafter, the slides are stained through Weigert's Iron Hematoxylin Solution, 0.05% Fast Green (FCF) Solution, 1% Acetic Acid Solution, and 0.1% Safranin O Solution. Mount the slides using resinous medium.
- Score the Safranin O-stained slides based on loss of proteoglycan (red color) in cartilage and percentage of destruction in cartilage structure, do statistical analysis for the histological score.
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