Materials
| Name | Company | Catalog Number | Comments |
| CMNB-caged fluorescein SE | Invitrogen | C20050 | |
| 10 kDa Aminodextran | Invitrogen | D1860 | |
| Zeba Desalt Spin Column | Pierce, Thermo Scientific | 89889 | |
| Low melting agarose | Amresco | 0815-100G | |
| Sodium Borate | Amresco | 1131117-100G | |
| Tricaine Methylsulfonate/ MS222 | Research Organics | 1347A | |
| Anti-fluorescein-AP | Roche Group | 11376622 | |
| Blocking buffer | Roche Group | 11 096 176 001 | |
| Maleic Acid | Sigma-Aldrich | MO375-500G | |
| Paraformaldehyde | Electron Microscopy Sciences | 15714 | |
| NBT | Sigma-Aldrich | I8377-250mg | |
| BCIP | Fisher Scientific | PI-34040 | |
| Microinjector | Harvard Apparatus | PLI-2000 | |
| LabTek chambered coverglass slides | Nalge Nunc international | 155380 | |
| Proteinase K | Invitrogen | AM2544 | |
| Lamb serum | Invitrogen | 16070096 | |
| LSM 510 META NLO | Carl Zeiss, Inc. | ||
| 20X 0.8 NA Air apochromatic objective | Carl Zeiss, Inc. | ||
| Transparent yellow filter | Theatre House Inc. | Roscolux filter sheet Color: 312 |
References
- Mathur, J. mEosFP-based green-to-red photoconvertible subcellular probes for plants. Plant physiology. 154, 1573-1587 Forthcoming.
- Wiedenmann, J. EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. Proceedings of the National Academy of Sciences of the United States of America. 101, 15905-15910 (2004).
- Subach, F. V. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature. 6, 153-159 (2009).
- Hatta, K., Tsujii, H., Omura, T. Cell tracking using a photoconvertible fluorescent protein. Nature protocols. 1, 960-967 (2006).
- Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H., Miyawaki, A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America. 99, 12651-12656 (2002).
- Watanabe, W. Single-organelle tracking by two-photon conversion. Optics express. 15, 2490-2498 (2007).
- Warga, R. M., Kane, D. A., Ho, R. K. Fate mapping embryonic blood in zebrafish: multi- and unipotential lineages are segregated at gastrulation. Developmental cell. 16, 744-755 (2009).
- Chudakov, D. M. Kindling fluorescent proteins for precise in vivo photolabeling. Nature. 21, 191-194 (2003).
- Habuchi, S., Tsutsui, H., Kochaniak, A. B., Miyawaki, A., van Oijen, A. M. mKikGR, a monomeric photoswitchable fluorescent protein. PloS ONE. 3, e3944-e3944 (2008).
- Nowotschin, S., Hadjantonakis, A. K. Use of KikGR a photoconvertible green-to-red fluorescent protein for cell labeling and lineage analysis in ES cells and mouse embryos. BMC developmental biology. 9, 49-49 (2009).
- Stark, D. A., Kulesa, P. M. An in vivo comparison of photoactivatable fluorescent proteins in an avian embryo model. Dev Dyn. 236, 1583-1594 (2007).
- Zhong, T. P., Childs, S., Leu, J. P., Fishman, M. C. Gridlock signalling pathway fashions the first embryonic artery. Nature. 414, 216-220 (2001).
- Vogeli, K. M., Jin, S. W., Martin, G. R., Stainier, D. Y. A common progenitor for haematopoietic and endothelial lineages in the zebrafish gastrula. Nature. 443, 337-339 (2006).
- Clanton, J. A., Shestopalov, I. A., Chen, J. K., Gamse, J. T. Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran. J. Vis. Exp. (50), e2672-e2672 (2011).
- Niemz, M. H. Laser-Tissue Interactions: Fundamentals and Applications. , Springer. (2003).
- Jowett, T.
