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Grote Plaats Milieu Genomic Library Production
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Grote Plaats Milieu Genomic Library Production

Article DOI: 10.3791/1387-v • 21:00 min • September 23rd, 2009
September 23rd, 2009 •
1, 1, 1, 1, 1
1Department of Microbiology and Immunology, University of British Columbia - UBC

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Bouw van een fosmid bibliotheek met milieu-genomisch DNA geïsoleerd uit de verticale diepte continuüm van een seizoensgebonden hypoxische fjord is beschreven. De resulterende kloon bibliotheek is geplukt in de 384-wells platen en gearchiveerd voor downstream-sequencing en functionele screening door de toepassing van een geautomatiseerd kolonie picking systeem.

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Basic protocollen milieu-genomische metagenomic genomisch DNA grote insert bibliotheek fosmid faag verpakking geautomatiseerde kolonie picking
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