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Harvesting and Digesting Mouse Skeletal Muscle for 2D Culture of Niche and Stem Cells (Video) | JoVE

Harvesting Mouse Skeletal Muscle to Culture Stem Cells and Their Niche

Article DOI: 10.3791/200241-v • 04:46 min • June 2nd, 2023
June 2nd, 2023 •
*1, *1, *1, 1, 1, 1, 1,2,3,4
1Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France, 2Ecole nationale vétérinaire d'Alfort, IMRB, F-94700 Maisons-Alfort, France, 3EFS, IMRB, F-94010 Creteil, France, 4AP-HP, Hopital Mondor, Service d'histologie, F-94010 Creteil, France
* These authors contributed equally

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Unfractionated Bulk Culture Mouse Skeletal Muscle Niche Stem Cell Quiescence Muscle Functionality Muscle Recovery Cell Types Molecular Signals Myofibers Muscle Stem Cells Physiological Microenvironment Ex Vivo Study Quiescent State Protocol 2D Culture Immunostaining Pax7-positive Cells Cell Amplification Generation Of Quiescent-like Stem Cells Fundamental Questions Translational Questions
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