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Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV) (Video) | JoVE

A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

Article DOI: 10.3791/200317-v • 03:08 min • August 18th, 2023
August 18th, 2023 •
1,2, 1,3,4,5, 1,6, 1, 1, 1,6, 7,8, 3,9, 1, 1,6
1School of Biodiversity, One Health & Veterinary Medicine, University of Glasgow, 2Research Institute for Tropical Medicine, 3University of Nairobi, Institute of Tropical and Infectious Diseases, 4Tanzania Industrial Research Development Organization, 5Ifakara Health Institute, 6MRC-University of Glasgow Centre for Virus Research, 7Department of Veterinary Public health, Ahmadu Bello University, 8African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, 9University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis, University of Nairobi

Tags

Whole Genome Sequencing Rapid Characterization Rabies Virus Nanopore Technology Genomic Data Transmission Tracking Geographic Spread Infectious Diseases Low- And Middle-income Countries (LMICs) Dog-mediated Rabies Vampire Bats Public Health Economic Concerns Sample-to-sequence-to-interpretation Workflow Nanopore Technology Sample Collection Rabies Diagnosis Whole Genome Sequencing Workflow Primer Design And Optimization Multiplex Polymerase Chain Reaction (PCR) Sequencing Library Preparation Live And Offline Base Calling Genetic Lineage Designation Phylogenetic Analysis Workflow Implementation Pipeline Validation Negative Controls Genomic Tools (GLUE MADDOG) Regional And Global Phylogenies
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