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Journal / Neuroscience / Mouse Brain Histone Modification Quantification… / Isolement de fragments enrichis par le système…
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JoVE Journal Neuroscience
Isolement de fragments enrichis par le système immunitaire du cerveau de souris pour la quantification des histones PTM
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Isolement de fragments enrichis par le système immunitaire du cerveau de souris pour la quantification des histones PTM

Mouse Brain Histone Modification Quantification Using Flow Cytometry

Article DOI: 10.3791/200980-v • 01:39 min • September 15th, 2023
September 15th, 2023 •
1, 2, 1
1Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health, University of British Columbia, 2Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health, University of British Columbia

Tags

Quantification Modifications post-traductionnelles des histones globales Cytométrie en flux intranucléaire Microglie cérébrale de souris isolée Contrôle de l’expression génique Structure de la chromatine Modifications post-traductionnelles Queues d’histones Acétylation Résidus de lysine Interactions avec l’ADN Accès aux facteurs de transcription Activateurs transcriptionnels contenant des bromodomaines Expression génique améliorée Régulation dynamique Différenciation cellulaire Environnements cellulaires Stimuli Approches de séquençage de nouvelle génération Haute Débit mesure quantitative
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