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Flowcytometrianalyse av celle-miRNA- og EV-miRNA-signal i elbiler isolert fra Calu6-celler transfektet med fluoroforkonjugert EV-miRNA

A Flow Cytometry Protocol for Understanding Export Mechanisms

Article DOI: 10.3791/201250-v • 03:01 min • October 6th, 2023
October 6th, 2023 •
1, 1,2
1Department of Biological Sciences, Purdue University, 2Purdue Institute for Cancer Research, Purdue University

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MiRNA-frigjøring ekstracellulære vesikler flowcytometri cellulær kommunikasjon bioaktive molekyler proteiner lipider nukleinsyrer mikroRNA ikke-kodende RNA mottakerceller last mikromiljø eksportdynamikk MiRNA sorteringsmekanismer protokoll flowcytometrianalyse EV-miRNA-lasting maskinidentifikasjon QRT-PCR transfeksjonskontroll
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