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JoVE Journal Biology
In situ Protocol for Butterfly Pupal Wings Using Riboprobes
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In situ Protocol for Butterfly Pupal Wings Using Riboprobes

Article DOI: 10.3791/208-v • 06:19 min • May 28th, 2007
May 28th, 2007 •
1, 2
1Department of Biological Sciences, SUNY-University at Buffalo, 2Dept. Ecology and Evolutionary Biology, Yale University

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In order to examine gene expression in the pupal wing tissue of Bicyclus anynana, we present an optimized protocol for in situ hybridizations using riboprobes. We also provide guidelines for the further optimization of this protocol for use in pupal wings of other Lepidopteran species.

Tags

In Situ Protocol Butterfly Pupal Wings Riboprobes In Situ Hybridizations Gene Expression Transcription Developmental Biology Spatial Patterns Temporal Patterns Antibodies Protein Products Larval Wing Discs Fragile Pupal Wings
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