Experiment
JoVE Encyclopedia of Experiments
Encyclopedia of Experiments: Biological Techniques
Encyclopedia of Experiments: Biological Techniques
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Reversed-Phase High-Performance Liquid Chromatography
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Reversed-Phase High-Performance Liquid Chromatography - ConceptTranscript
Analyze the samples, using a standard HPLC system connected to an online fluorescence detector. Use a reversed-phase C-18 column with a 250-millimeter length and 4.6-millimeter diameter for the analysis.
After preparing solvent A and solvent B, and setting up the HPLC run as detailed in the text protocol, inject 50 microliters of the sample into the HPLC system.
Monitor eluents, using the fluorescence detector excitation wavelength of 373 nanometers, and an emission wavelength of 448 nanometers.
Finally, calculate the relative amount of Neu5Gc as described in the text protocol.
