Encyclopedia of Experiments: Immunology
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Transcript
To test serum samples for serum immunoglobulin E antibodies to whole allergens, randomly select three samples with at least 900 microliters of serum, and take the chip from the kit, and number it. Then, turn on the computer and the analyzer power. Start the LabIT program on the computer.
If the dark frame warning window pops up, click OK to run a leak test. Then, click the center logo to enter the operation interface. Check the reaction temperature and the charge-coupled device or CCD temperature. The reaction temperature should rise to 37 plus or minus 1 degrees Celsius in about 10 minutes, and the CCD temperature should drop to minus 15 plus or minus 1 degrees Celsius.
When the CCD temperature has dropped to minus 15 plus or minus 1 degrees Celsius, confirm that there are no other items left inside the instrument and close the door. Then click Tools, System Test, Leak Test. When the test is finished, the report window will pop up.
Next, load 620 microliters of wash buffer, 120 microliters of blocking buffer, 60 microliters of conjugates A and B, 60 microliters of substrates A and B, and 100 microliters of each serum sample into the corresponding reagent tanks on the microfluidic cartridge. After clicking cartridge ID, use the barcode scanner to scan the serial number of the cartridge and enter the sample ID. Then, place the cartridge into the analyzer, close the door, and click analyzer, and run to start the analysis.
