JoVE Journal
Neuroscience
Neuroscience
This content is Open Access.
Chapters
Summary
Please note that all translations are automatically generated.
This protocol outlines the steps required to dissect, transfect via electroporation and culture mouse hippocampal and cortical neurons. Short-term cultures may be used for studies of axon outgrowth and guidance, while long-term cultures can be used for studies of synaptogenesis and dendritic spine analysis.