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Journal / Neuroscience / Análise do transcriptoma de uma única célula
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Neuroscience

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JoVE Journal Neuroscience
Análise do transcriptoma de uma única célula
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Análise do transcriptoma de uma única célula

Article DOI: 10.3791/2634-v • 07:27 min • April 25th, 2011
April 25th, 2011 •
*1, *1, 1,2
1Department of Pharmacology, University of Pennsylvania, 2The Penn Genome Frontiers Institute, University of Pennsylvania
* These authors contributed equally

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Neste artigo descrevemos um método simples para a colheita de células isoladas de ratos culturas primárias neuronais e análise de transcriptoma subsequentes usando a amplificação ARNA. Esta abordagem é generalizável a qualquer tipo de célula.

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Neurociência Edição 50 de uma única célula transcriptoma amplificação Arna RT-PCR biologia molecular expressão gênica
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