Name: Experimental Approaches to Tissue Engineering (Video) | JoVE Journal
Uploaded: 2007-08-30T00:00:00.000+00:00
Duration: 16 min 41 s
Description: Learn microscale tissue engineering approaches for regenerative medicine using stem cell differentiation. This method helps researchers address organ shortage and develop better drug discovery models.

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experimental approaches to tissue engineering

Experimental Approaches to Tissue Engineering

My name is Ali Kamasi and I am currently a assistant professor at the Harvard MIT's Division of Health Sciences and Technology. And I have a joint appointment with Harvard Medical School, specifically at Brigham and Women's Hospital, which is a affiliate of Harvard Medical School. And my research areas are in microscale tissue engineering and biomes area.So my background academically is more to, and initially started in chemical engineering. I did my undergraduate in chemical engineering at University of Toronto, and subsequently I became very interested in more biology oriented engineering. So I did a master's in biomedical engineering continuing in University of Toronto, working specifically in stem cell differentiation.And then I, I came to MIT and I did my PhD with Professor of Robert Langer in, in, in bioengineering. And afterwards, after a few months of postdoc, I joined my current position. The research areas that we focus on is really at the interface between biology, engineering and material science.So, and what we are trying to do is to try to address questions that bio biologists have been trying to answer for a long time, but try to do it in a way that previously could

experimental-approaches-to-tissue-engineering

Research

JoVE Journal

Biology

Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.

Tissue Engineering of a Human 3D in vitro Tumor Test System

For creation of highly organized structures of complex tissue, one must assemble multiple material and cell types into an integrated composite.  This combinatorial design incorporates organ-specific layered cell sheets with two distinct biologically-derived materials containing a strong fibrous matrix base, and endothelial cells for enhancing new vessels formation.

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Tissue engineering often includes in vitro expansion in order to create autografts for tissue regeneration. In this study a method for tissue expansion, regeneration, and reconstruction in vivo was developed in order to minimize the processing of cells and biological materials outside the body.

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

A Cartesian bioprinter was designed and fabricated to allow multi-material deposition in precise, reproducible geometries, while also allowing control of environmental factors. Utilizing the three-dimensional bioprinter, complex and viable constructs may be printed and easily reproduced.

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

This is a guideline for constructing in vivo vascularized tissue using a microsurgical arteriovenous loop or a flow-through pedicle configuration inside a tissue engineering chamber. The vascularized tissues generated can be employed for organ regeneration and replacement of tissue defects, as well as for drug testing and disease modeling.

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

We provide a generalized protocol based on a microfluidic bioprinting strategy for engineering a microfibrous vascular bed, where a secondary cell type could be further seeded into the interstitial space of this microfibrous structure to generate vascularized tissues and organoids.

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

This protocol describes a platform for fabricating self-assembled tissue rings in variable sizes using a customized 3D-printed plastic mold. PDMS negatives are cured in the 3D-printed mold; then agarose is cast in the cured PDMS negatives. Cells are seeded into the resulting agarose wells where they aggregate into tissue rings.

Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds

Presented here is a simple-to-use, core/shell, three-dimensional bioprinting set-up for one-step fabrication of hollow scaffolds, suitable for tissue engineering of vascular and other tubular structures.

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Presented here is a protocol to generate engineered connective tissues for a parallel culture of 48 tissues in a multi-well plate with double poles, suitable for mechanistic studies, disease modeling, and screening applications. The protocol is compatible with fibroblasts from different organs and species and is exemplified here with human primary cardiac fibroblasts.

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Learn microscale tissue engineering approaches for regenerative medicine using stem cell differentiation. This method helps researchers address organ shortage and develop better drug discovery models.

Experimental Approaches to Tissue Engineering

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