Biology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Please note that all translations are automatically generated.
An in vitro method for preparing functional glucocorticoid receptor (GR)•hsp90 protein complexes from purified proteins and cellular lysates is described. The method utilizes immunoadsorption of recombinant GR followed by salt-stripping and protein complex reconstitution. The importance of cofactors and buffer conditions are discussed, as are potential method applications.