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Journal / Biology / 一个定量测定胰岛素表达细胞集落形成先觉…
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一个定量测定胰岛素表达细胞集落形成先觉
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一个定量测定胰岛素表达细胞集落形成先觉

Article DOI: 10.3791/3148-v • 14:39 min • November 28th, 2011
November 28th, 2011 •
1, 2, 2, 2, 2, 2, 2,3
1Department of Biotechnology & Bioinformatics, California State University Channel Islands, 2Department of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of City of Hope, 3The Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope

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描述一个立体的克隆形成实验，让胰祖细胞分化为胰岛素表达殖民地。此方法采用半固体甲基纤维素，基质胶和生长因子的媒体的优势，其中单祖细胞增殖和分化

Tags

发育生物学，57期，胰腺，胰岛素表达细胞，胚胎干细胞，菌落检测，祖细胞，三维培养，半固体培养基，基质胶，甲基纤维素
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