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High-throughput Zuivering van Affinity gemerkte recombinante eiwitten
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High-throughput Zuivering van Affinity gemerkte recombinante eiwitten

Article DOI: 10.3791/4110-v • 07:45 min • August 26th, 2012
August 26th, 2012 •
1, 1
1Department of Life Sciences, Imperial College London

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We beschrijven een werkwijze voor de affiniteit-gemerkte zuivering van recombinante eiwitten met vloeistof handling robotica. Deze methode is algemeen toepasbaar op de kleinschalige zuivering van oplosbare His-gemerkte eiwitten in een high-throughput format.

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Biochemie Genetica Moleculaire Biologie Bio-informatica recombinante eiwitten histidine-tag affiniteitszuivering high-throughput automatisering
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