JoVE Journal
Immunology and Infection
Immunology and Infection
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Chapters
Summary
Please note that all translations are automatically generated.
This protocol describes the use of peptide:MHC tetramers and magnetic microbeads to isolate low frequency populations of epitope-specific T cells and analyze them by flow cytometry. This method enables the direct study of endogenous T cell populations of interest from in vivo experimental systems.