Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Analysis of the Solvent Accessibility of Cysteine …
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
Kupffer Cell Isolation for Nanoparticle Toxicity Testing
Journal (Immunology and Infection)
Kupffer Cell Isolation for Nanoparticle Toxicity Testing
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
Journal (Immunology and Infection)
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
 

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

Article DOI: 10.3791/50084-v • 08:27 min • February 14th, 2013
February 14th, 2013 •
1,2, 1,2
1Plant Sciences Institute, Agricultural Research Service, United States Department of Agriculture, 2Molecular Plant Pathology Laboratory, Agricultural Research Service, United States Department of Agriculture

Chapters

Summary
Automatic Translation

A method to analyze the solvent accessibility of the thiol group of cysteine residues of Maize rayado fino virus (MRFV)-virus-like particles (VLPs) followed by a peptide cross-linking reaction is described. The method takes advantage of the availability of several chemical groups on the surface of the VLPs that can be targets for specific reactions.

Tags

Solvent Accessibility Cysteine Residues Maize Rayado Fino Virus Virus-like Particles Nicotiana Benthamiana Plants Cross-linking Of Peptides Virus Properties Physicochemical Characteristics Physical Characteristics Virus-based Technologies Self-assembly Biomaterials Vaccines Electronic Materials Chemical Tools Molecular Electronic Containers Nanotechnology Genetic Modification Chemically Attaching Molecules Virus Particle Reactive Groups
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter