JoVE Journal
Neuroscience
Neuroscience
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.