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Isolering av Neonatal Ekstrahepatisk Cholangiocytes

Article DOI: 10.3791/51621-v • 07:54 min • June 5th, 2014
June 5th, 2014 •
1, 2
1Division of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, 2Department of Medicine, The Perelman School of Medicine at the University of Pennsylvania

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En teknikk for å isolere cholangiocytes fra de ekstrahepatiske gallegangene av neonatal mus er beskrevet. Kanalene er omhyggelig dissekert, og cellene blir isolert ved utvekst i tykke kollagen geler. Denne metoden gir et nyttig verktøy for å studere ekstrahepatiske gallegang utvikling og patologi.

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