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Studerer DNA Looping av Single-Molecule FRET
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Studerer DNA Looping av Single-Molecule FRET

Article DOI: 10.3791/51667-v • 11:27 min • June 28th, 2014
June 28th, 2014 •
1, 1
1School of Physics, Georgia Institute of Technology

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Denne studien presenterer en detaljert eksperimentell prosedyre å måle looping dynamikken i dobbel-strandet DNA ved hjelp av single-molekyl fluorescens Resonance Energy Transfer (FRET). Protokollen beskriver også hvordan du kan hente looping sannsynlighetstetthet kalt J-faktor.

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Molecular Biology DNA looping J-faktor enkelt molekyl FRET Gel mobilitet skift DNA kurvatur Worm-lignende kjede
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