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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Article DOI: 10.3791/51845-v • 11:48 min • October 9th, 2014
October 9th, 2014 •
*1, *1, 1
1Division of Hematology/Oncology, Department of Medicine, Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine
* These authors contributed equally

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Alternative splicing regulation has been shown to contribute to the epithelial-mesenchymal transition (EMT), an essential cellular program in various physiological and pathological processes. Here we describe a method utilizing an inducible EMT model for the detection of alternative splicing during EMT.

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Alternative Splicing Epithelial-mesenchymal Transition EMT Detection Strategy Inducible EMT Model Transcription Repressor Twist Cell Morphology E-cadherin Junctions EMT Markers Epithelial Markers Mesenchymal Markers Isoform-specific Primer Sets Quantitative RT-PCR Protein Isoforms Immunoblotting Assays Biological Processes
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