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Journal / Neuroscience / Multi-photon Intracellular Sodium Imaging…
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Neuroscience

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JoVE Journal Neuroscience
Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
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Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Article DOI: 10.3791/52038-v • 10:29 min • October 8th, 2014
October 8th, 2014 •
*1, *1, 1
1Institute of Neurobiology, Heinrich Heine University Düsseldorf
* These authors contributed equally

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Summary
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We describe the combination of focal UV-induced photo-activation of neuro-active compounds with whole-cell patch-clamp and multi-photon imaging of intracellular sodium transients in dendrites and spines of hippocampal neurons in acute tissue slices of the mouse brain.

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Multi-photon Intracellular Sodium Imaging UV-mediated Focal Uncaging Glutamate CA1 Pyramidal Neurons Fluorescence Microscopy Morphological Parameters Physiological Parameters Brain Cells Spatial Resolution Temporal Resolution Electrophysiology Calcium Signals Subcellular Compartments Dendrites Dendritic Spines Synaptic Activity Postsynaptic Sodium Signals Whole-cell Patch-clamp Micro Domains Central Neurons UV-induced Uncaging Glutamate Receptors Cornu Ammonis Subdivision 1 (CA1) Acute Tissue Slices Mouse Hippocampus Sodium-sensitive Fluorescent Dye SBFI
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