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Journal / Bioengineering / The Multi-organ Chip - A Microfluidic Platform…
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Bioengineering

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JoVE Journal Bioengineering
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The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

Article DOI: 10.3791/52526-v • 10:05 min • April 28th, 2015
April 28th, 2015 •
*1, *1,2, 1, 1,2, 1, 3, 3, 1, 1,2
1Medical Biotechnology, Technische Universität Berlin, 2TissUse GmbH, 3Fraunhofer IWS
* These authors contributed equally

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Summary
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Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.

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Multi-organ Chip Microfluidic Platform Long-term Multi-tissue Coculture Substance Testing In Vitro Test Systems Drug-induced Liver Injury Microenvironment Multiple Dosing Experiments Extracellular Conditions Microengineered Bioreactor On-chip Pump Culture Spaces Circulatory System Primary Cell Cultures Human Liver Equivalents HepaRG Cells Hepatic Stellate Cells Hanging Drop Plates Human Skin Punch Biopsies Microbioreactor System
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