Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Silencing of BRCA2 to Identify Novel…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
Journal (Biology)
Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
Capture Compound Mass Spectrometry - A Powerful Tool to Identify Novel c-di-GMP Effector Proteins
Journal (Biology)
Capture Compound Mass Spectrometry - A Powerful Tool to Identify Novel c-di-GMP Effector Proteins
 

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Article DOI: 10.3791/52849-v • 09:24 min • August 12th, 2015
August 12th, 2015 •
1, 1, 1
1Institute of Biomembranes and Bioenergetics, National Research Council

Chapters

Summary
Automatic Translation

Gene silencing by siRNA represents a convenient experimental strategy to analyze BRCA2-dependent biological functions with immediate implications to better understand cancer biology. A method to efficiently silence BRCA2, along with the experimental procedure to detect and quantify changes in BRCA2 protein expression by immunoblotting in human cell lines, is presented.

Tags

BRCA2 Silencing Tumor Suppressor Protein SiRNA Transfection Immunoblotting Human Epithelial Cell Lines Gene Silencing Protein Detection Immunoblotting Analysis BRCA2 Mutations Cellular Functions
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter