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Immunology and Infection

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JoVE Journal Immunology and Infection
Organoids as Model for Infectious Diseases
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Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori

Article DOI: 10.3791/53359-v • 10:30 min • November 12th, 2015
November 12th, 2015 •
1, 1
1Hubrecht Institute for Developmental Biology and Stem Cell Research, University Medical Centre Utrecht

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Summary
Automatic Translation

Stem cell derived cultures harbor tremendous potential to model infectious diseases. Here, the culture of mouse and human gastric organoids derived from adult stem cells is described. The organoids are microinjected with the gastric pathogen Helicobacter pylori.

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Organoids Model Infectious Diseases Culture Human Murine Stomach Organoids Microinjection Helicobacter Pylori Infection Biologists Stem Cells Embryonic Stem Cells Induced Pluripotent Stem Cells Adult Stem Cells Gastric Glands Basement Matrix Growth Factors Epidermal Growth Factor (EGF) R-spondin Noggin Wnt Fibroblast Growth Factor (FGF) 10 Gastrin Transforming Growth Factor (TGF) Beta Inhibitor 3-dimensional Organoids Lineages Of The Stomach Indefinitely Expandable Organoids Frozen And Thawed Organoids Bacteria Infection Studies
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