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Real-time Imaging of Plant Cell Surface Dynamics med variabel vinkel epifluorescens Mikros
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Real-time Imaging of Plant Cell Surface Dynamics med variabel vinkel epifluorescens Mikros

Article DOI: 10.3791/53437-v • 06:31 min • December 12th, 2015
December 12th, 2015 •
1
1Graduate School of Frontier Sciences, The University of Tokyo

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Målet med denne protokollen er å vise hvordan du kan overvåke fluorescently-merkede protein dynamikk på plantecelleoverflater med variabel vinkel epifluorescence mikroskopi, viser blinkende prikker av GFP-merket PATROL1, en membran trafficking protein, i cellen cortex av stomatal kompleks i Arabidopsis thaliana.

Tags

Plant Biology , eksocytose Fluorescent proteiner bildeanalyse Kymograph Membran trafficking Plant cellebiologi Real-time imaging stomata Total intern refleksjon mikroskopi Variable-vinkel epifluorescence mikros
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